M A Collings

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Methods were recently developed for culturing neurons from adult rat hippocampus using the serum-free medium Neurobasal with B27 supplement. To determine whether adult cultured neurons have normal electrical properties, we studied cultures from rats of three age groups: (1) embryonic; (2) 10-11 months old and (3) 35-36 months old. Neurons had a polarized(More)
Calcium channels affect many neuronal functions, including membrane electrical excitability, synaptic transmission, cellular homeostasis, gene transcription, growth, and development. We used recently developed methods for serum-free culture of adult and embryonic rat neurons to study the development of voltage-sensitive calcium currents. We compared(More)
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