Mélanie HUBERT

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F11 is a glycosyl phosphatidylinositol-anchored axonal surface glycoprotein belonging to a neural subgroup of the immunoglobulin superfamily. In this report, we demonstrate that the F11 protein displays three distinguishable activities: binding to the cell recognition molecule Ng-CAM, interaction with the extracellular matrix glycoprotein restrictin (RN),(More)
F11 and Nr-CAM/Bravo are two axon-associated glycoproteins belonging to different subgroups of the immunoglobulin superfamily. In this report we have investigated the interaction of both proteins using neurite outgrowth and binding assays. Antibody blocking experiments demonstrate that neurite extension of tectal cells on immobilized F11 is mediated by(More)
The extracellular matrix glycoprotein tenascin-R (TN-R) is a multidomain protein implicated in neural cell adhesion. To analyze the structure-function relationship of the different domains of TN-R, several recombinant TN-R fragments were expressed in bacterial cells. Two distinct binding regions were localized on the TN-R polypeptide: a region binding the(More)
Tenascin-R (TN-R) is an extracellular matrix protein associated with the surface of neurons and glial cells. Immunohistological studies reveal that TN-R shows a restricted expression pattern in the developing nervous system. TN-R is the smallest member of the tenascin family and is composed of four structural motifs: a cysteine-rich segment at the(More)
KEY WORDS: CHROMOSOME / CHO CELLS / METAPHASE / KARYOTYPE / DIGITAL IMAGING BACKGROUND AND NOVELTY: Chinese hamster ovary (CHO)-derived cells are widely used for the production of recombinant proteins. Currently, the pharmaceutical industry relies on stable cell clones for therapeutic protein production. Most of the currently used methods to analyze(More)
A major problem for the pharmaceutical industry is the failure of promising drug candidates very late in the testing phases. Often, there are unpredicted side-effects and toxicity issues which limit or prevent a candidate molecule being taken to market. Any testing regimes that can identify toxic molecules and exclude them from the screening programs as(More)
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