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Encephalitozoonosis is an opportunistic infection in animals and humans. Its clinical form is observed in immunosuppressed hosts. We studied the occurrence of the manifest form of rabbit microsporidiosis under cyclophosphamide immunomodulation in 40 New Zealand rabbits. The experimental animals were intraperitoneally infected with 5 x 10(7) Encephalitozoon… (More)
The development of genetic methods broadens the scope of non-invasive sample types. Large shed feathers are good material for genetic analyses, since they are easy to collect and a single feather can provide a sufficient amount of DNA for PCR-based methods. Previous studies have demonstrated that feather quality and type affect the DNA quality extracted… (More)
The SH-groups of alpha-mercapto-propionylglycine (MEPRIN) are remarkably stable in diluted solution either at room temperature or at 50 degrees C, even if storing takes place under air-bubbling. The SH-activity was measured by polarographic method and amperometric titration. Neutralization of the carboxyl group decreases the stability of the sulfhydryl… (More)
The cytoplasm of normal and tumorous rat liver cells contains a heat-resistant active compound with reducing ability to break the mixed disulfide bond of albumin-14C-mercaptoethylguanidine. The reducing activity of cytosol is destroyed by 1000 krd 60Co-gamma-ray doses in diluted solution. In vivo supralethal irradiation of rats does not affect the activity… (More)
A theoretical assumption of Eldjarn and Pihl suggests that mixed disulphides formed by radioprotective aminothiols and protein SH-groups can be broken down by enzymes in the organism, and the native structure of the macromolecules restored. Irradiation should enhance this effect. In our experiments, mixed disulphides of mercaptoethylguanidine (MEG) and… (More)
This disease occurred in 6 rabbits in which leukemic changes after infection with the bovine leukemia virus were being studied.