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The responsiveness of glycogen breakdown to cAMP was investigated in isolated perfused liver from male Wistar fed rats (200-220 g) with insulin-induced hypoglycemia. The activation of glycogenolysis by 3 microM cAMP was decreased (P<0.05) in livers from rats with hypoglycemia induced by the administration of insulin or during the direct infusion of insulin… (More)
Hepatic responsiveness to gluconeogenic substrates during insulin-induced hypoglycemia was investigated. For this purpose, livers were perfused with a saturating concentration of 2 mM glycerol, 5 mM L-alanine or 5 mM L-glutamine as gluconeogenic substrates. All experiments were performed 1 h after an ip injection of saline (CN group) or 1 IU/kg of insulin… (More)
The time-course changes of the responsiveness of glycogen breakdown to alpha- and ss-adrenergic agonists during insulin-induced hypoglycemia (IIH) were investigated. Blood glucose levels were decreased prior to the alteration in the hepatic responsiveness to adrenergic agonists. The activation of hepatic glucose production and glycogenolysis by… (More)
Leptin showed less prominent inhibiting effect on the activation of hepatic glycogen breakdown and gluconeogenesis promoted by cAMP. The role of cAMP in the inhibition of glycogen breakdown and gluconeogenesis induced by physiological levels of leptin (10 ng/ml) and insulin (20 microU/ml) in the perfused liver was investigated. Insulin but not leptin… (More)
AIM To investigate the hepatic capacity to produce glucose during hypoglycemia induced by insulin (HII). METHODS Livers from 24-h fasted rats which received i.p. insulin (HII rats) or saline (control rats) were perfused in situ. The gluconeogenic substrates L-alanine (5 mmol/L), L-glutamine (5 mmol/L), L-lactate (2 mmol/L), and glycerol (2 mmol/L) were… (More)
Physiological level of insulin showed more prominent inhibiting effect on the activation of hepatic glucose production and glycogenolysis promoted by cAMP than physiological levels of leptin.