Lyudmila P. Simova-Stoilova

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The leaf protein pattern from drought-tolerant and drought-sensitive wheat varieties subjected to severe soil drought but with the possibility for recover from stress was studied by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The spots representing Rubisco large subunit (RLS) were identified using polyclonal antibodies against Rubisco and(More)
The involvement of acidic proteases in soil drought response of winter wheat (Triticum aestivum L.) at seedling stage in three cultivars differing in water stress tolerance was studied. Withholding irrigation for seven days resulted in severe drought stress corresponding to 60% leaf water deficit. Stressed plants were recovered by providing optimal water(More)
529 Drought stress is an important environmental factor constraining crop productivity. Wheat is essential nourishment for more than 1/3 of the world population and crop yield will be considerably influenced in the perspective of global climate change and limitation of water resources in the environment (Chaves and Oliveira 2004). Much of the injury on(More)
Holm oak is a dominant tree in the western Mediterranean region. Despite being well adapted to dry hot climate, drought is the main cause of mortality post-transplanting in reforestation programs. An active response to drought is critical for tree establishment and survival. Applying a gel-based proteomic approach, dynamic changes in root proteins of(More)
Antioxidant response to drought in red (Trifolium pratense L., cv. “Start”) and white clover (Trifolium repens L, cv. “Haifa” and cv. “Debut”) grown as soil cultures was evaluated in water-deprived and recovered plants. Drought provoked oxidative stress in leaves confirmed by the considerable changes in electrolyte leakage, malondialdehyde, hydrogen(More)
The main objective of the present work was to examine leaf respiratory responses to dehydration and subsequent recovery in three varieties of winter wheat (Triticum aestivum L.) known to differ in their level of drought tolerance. Under dehydration, both total respiration and salicylhydroxamic acid (SHAM)-resistant cytochrome (Cyt) pathway respiration by(More)
Wheat plants with a fully developed first leaf were subjected to severe but recoverable water stress. Leaves from drought tolerant (Katya and Zlatitza) and drought sensitive (Sadovo and Miziya) varieties in control, drought and recovery conditions were used for the experiments. The physiological response of drought tolerant varieties did not differ from the(More)
Intracellular proteases could be involved in stress induced protein degradation and metabolism reorganization. Data on this topic are still quite limited. Eight varieties of winter wheat (Triticum aestivum L.) with different field drought resistance were examined in this study. Plants were grown as soil cultures in a growth chamber and watered daily to(More)
Three varieties (cv. Pobeda, Katya and Sadovo) of winter wheat (Triticum aestivum), differing in their agronomic characteristics, were analysed during progressive soil water stress and recovery at early vegetation stages. Changes in abscisic acid content, SDS-PAGE and immunoblot profiles of proteins that remained soluble upon heating were monitored.(More)
A comparative study of the response to waterlogging in a tolerant (Trifolium repens L., white clover cultivar Rivendel) and susceptible (Trifolium pratense L., red clover cultivar Raya) plants was undertaken to reveal the possible link between plant performance and oxidative stress protection mechanisms in leaves. Two weeks of soil waterlogging induced(More)