Lynn E. Fletcher

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BACKGROUND We have compared mutation analysis by DNA sequencing and Amplification Refractory Mutation System™ (ARMS™) for their ability to detect mutations in clinical biopsy specimens. METHODS We have evaluated five real-time ARMS assays: BRAF 1799T>A, [this includes V600E and V600K] and NRAS 182A>G [Q61R] and 181C>A [Q61K] in melanoma, EGFR 2573T>G(More)
OBJECTIVE To assess the effectiveness of antibiotic treatment of women with symptoms of urinary tract infection but negative urine dipstick testing. DESIGN Prospective, double blind, randomised, placebo controlled trial. SETTING Primary care, among a randomly selected group of general practitioners in Christchurch, New Zealand. PARTICIPANTS 59 women(More)
OBJECTIVES To compare the efficacy, safety, and acceptability of treatment with intravenous antibiotics for cellulitis at home and in hospital. DESIGN Prospective randomised controlled trial. SETTING Christchurch, New Zealand. PARTICIPANTS 200 patients presenting or referred to the only emergency department in Christchurch who were thought to require(More)
BACKGROUND Event-based measures suggest that emotional adversity in childhood has a long-term health impact, but less attention has been paid to chronic emotional stressors such as family conflict, harsh discipline or lack of affection. This study aimed to assess the impact of the latter on health problems and illness in adulthood. METHODS Logistic(More)
BRAF mutation testing from circulating free DNA (cfDNA) using the amplification refractory mutation testing system (ARMS) holds potential as a surrogate for tumor mutation testing. Robust assay validation is needed to establish the optimal clinical matrix for measurement and cfDNA-specific mutation calling criteria. Plasma- and serum-derived cfDNA samples(More)
The cherry leaf roller (Caloptilia serotinella) produces three distinct types of substrate-borne signals—scraping, plucking, and vibrating—during interactions between conspecifics. Signals were recorded using a piezoelectric sensor, and behavioral experiments tested the hypothesis that signaling functions in territorial disputes over costly leaf shelters.(More)
Group living confers both benefits and costs to the individuals involved. Benefits may include enhanced defense, thermoregulation, and increased foraging efficiency while costs often involve competition for resources such as food, shelter, and mates. Communication provides a medium of exchange among individuals engaged in either cooperative or competitive(More)
During periods of travel or dispersive activities (e.g., foraging), group-living animals face the common challenge of maintaining a cohesive unit. At the basic level, this challenge is no different for vertebrates than it is for arthropods and is solved through communication. Gregarious larvae of the Australian sawfly, Perga affinis, communicate via(More)
BACKGROUND Prevalence estimates of chronic disease vary according to the technique used. Questionnaire surveys may be susceptible to inaccuracies, which may be overcome by addition of a checklist of conditions. This paper presents SF-36 scores and NHS consultation rates for people reporting individual chronic diseases or disabilities in two questionnaire(More)
AIM To examine the incidence of thick melanoma in New Zealand from 1994-2004 and investigate associations with melanoma thickness. METHOD The New Zealand Health Information Service provided information on all registrations for malignant melanoma from 1994-2004. Age-standardised registration rates were calculated. Logistic regression analysis was(More)