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Sharply delineated domains of cell types arise in developing tissues under instruction of inductive signal (morphogen) gradients, which specify distinct cell fates at different signal levels. The translation of a morphogen gradient into discrete spatial domains relies on precise signal responses at stable cell positions. However, cells in developing tissues(More)
Epithelial cells acquire functionally important shapes (e.g., squamous, cuboidal, columnar) during development. Here, we combine theory, quantitative imaging, and perturbations to analyze how tissue geometry, cell divisions, and mechanics interact to shape the presumptive enveloping layer (pre-EVL) on the zebrafish embryonic surface. We find that, under(More)
In analysis of microscopy based images, a major challenge lies in splitting apart cells that appear to overlap because they are too densely packed. This task is complicated by the physics of the image acquisition that causes large variations in pixel intensities. Each image typically contains thousands of cells with each cell having a different orientation,(More)
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