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A cellulase gene (cel28a) was isolated from a rumen microbial metagenome library of goat rumen microorganisms, cloned into E. coli, and expressed in active form. The gene has a length of 1596 bp obtained using a genome walking Kit and encodes a protein of 509 amino acids with a calculated MW of 55 kDa. The deduced amino acid sequence was homologous with(More)
Background: Porcine epidemic diarrhea (PED) is a highly contagious disease of pigs, and is characterized by a series of clinical symptoms, such as severe diarrhea, vomiting and dehydration. Partial protective antigen gene (COE gene) of S protein possessing the main B cell epitope, is able to encode proteins with reactogenicity to induce the production of(More)
The neonatal Fc receptor (FcRn) transports IgG from mother to young and is involved in antigen presentation. FcRn is structurally similar to MHC class I, but its intracellular trafficking pathway is much more analogous to that of MHC class II. Ia-associated invariant chain (Ii) molecules play an additional role in directing MHC class II trafficking within(More)
OBJECTIVE To isolate thermophilic bacteria to degrade organic substances of dead-pig. METHODS Primary screening was done by using diluted plate count and selective medium, and then enzyme activity was measured for secondary screening. Two thermophilic bacterial strains N-3 and Y-3 were isolated, and could degrade protein and lipids. To test their effect,(More)
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