Luziane M Bendhack

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The contribution of endothelial factors and mechanisms underlying decreased acetylcholine-induced relaxation and endothelial inhibitory action on phenylephrine-induced contraction were evaluated in aortas of two-kidney, one-clip hypertensive (2K-1C) and normotensive (2K) rats. Relaxation induced by acetylcholine in 2K-1C precontracted by phenylephrine was(More)
S-nitroso-glutathione (GSNO) and S-nitroso-N-acetylcysteine (NACysNO) are nitrosothiols that release nitric oxide (NO) and mimic the effects of endogenous NO. This study investigated the relaxation induced by GSNO and NACysNO in rat aorta and the relation between relaxation and NO formation. Both compounds at concentrations from 10(-9) M to 10(-4) M relaxed(More)
This study examined whether alterations in intracellular or extracellular Ca2+ mobilization were related to differences in caffeine and phenylephrine (PHE)-induced contractions between two-kidney. one-clip hypertensive (2K-1C) and normotensive (2K) rat aortas. After depletion and reloading of intracellular Ca2+ stores, caffeine and PHE-induced contractions(More)
The previous finding that converting enzyme inhibitors (CEIs) potentiate bradykinin (BK), but do not inhibit conversion of angiotensin (ANG) I in isolated vessels, was explored further in the rat perfused mesenteric vascular bed. To investigate whether other peptidases besides angiotensin converting enzyme (ACE) might be involved in CEI-resistant ANG I(More)
Confocal microscopy has contributed to important findings in biology and medicine, because it enables the acquisition of fluorescence images of tissues and cells. Regarding vascular disorders, this technique has become an indispensable tool for the clarification of functions in vascular biological systems through the utilization of specific fluorescent(More)
The hypothesis that signal transduction mediated by platelet-derived growth factor (PDGF) and angiotensin II (Ang II) is altered in vascular smooth muscle (VSM) cells from the spontaneously hypertensive rat (SHR) was tested by measuring changes in the cytosolic free calcium concentration ([Ca2+]i). [Ca2+]i was measured in cultured aortic smooth muscle cells(More)
Stimulation of phosphatidylinositol metabolism by neurotransmitters produces diacylglycerol, an activator of protein kinase C, which may be involved in hormone-mediated contractions. We studied the effect of a tumor-promoting phorbol ester, 12-deoxyphorbol 13-isobutyrate 20-acetate (DPBA), on contraction of caudal artery rings of Wistar-Kyoto control (WKY)(More)
1. The aims of the present study were to investigate the contribution of intracellular calcium and to evaluate the effect of the antagonists of the intracellular calcium stores, thapsigargin and [8-(Diethylamino)-octyl-3,4,5-trimethoxybenzoate, HC1] TMB-8, on phenylephrine-stimulated contractions of rat anococcygeus smooth muscle, using functional studies.(More)
In the rat anococcygeus muscle both dopamine and noradrenaline induced concentration-dependent contractile responses. The alpha 1-antagonist prazosin inhibited both dopamine and noradrenaline responses, whereas the alpha 2-antagonist yohimbine influenced noradrenaline-mediated responses only. The concentration-effect curves for dopamine were shifted to the(More)
Irradiation of trans-[RuCl(cyclam)(NO)](2+), cyclam is 1,4,8,11-tetraazacyclotetradecane, at pHs 1-7.4, with near UV light results in the release of NO and formation of trans-[Ru(III)Cl(OH)(cyclam)](+) with pH dependent quantum yields (from approximately 0.01 to 0.16 mol Einstein(-1)) lower than that for trans-[RuCl([15]aneN(4))(NO)](2+), [15]aneN(4) is(More)