Lukáš Maršálek

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Sustainable production of microbial polyhydroxyalkanoate (PHA) biopolyesters on a larger scale has to consider the "four magic e": economic, ethical, environmental, and engineering aspects. Moreover, sustainability of PHA production can be quantified by modern tools of Life Cycle Assessment. Economic issues are to a large extent affected by the applied(More)
In field trials conducted over three growth seasons I studied the differences in activities of the enzymes: polyphenoloxidase, ascorbateoxidase and peroxidase, with a heterotic single-cross hybrid of maize and its pollen-sterile analogue. Additional observations were made for differences between the single-cross hybrids and their parental lines. The samples(More)
Polyacrylamide gel electrophoresis was used to study the differences in patterns of the isoperoxidase spectrum in leaf tissues of the genome series (2n, 3n, 4n) of twoNicotiana tabacum L. cultivars,i.e. in the growth stage of the 5th–6th and 10th–11th leaf, and in the stage of elongation growth. Mutual comparisons of the cultivars showed that the(More)
The methylotrophic yeast Pichia pastoris (Komagataella spp.) is a popular microbial host for the production of recombinant proteins. Previous studies have shown that mis-sorting to the vacuole can be a bottleneck during production of recombinant secretory proteins in yeast, however, no information was available for P. pastoris. In this work the authors have(More)
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