Luis Z. Avila

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This paper describes the estimation of binding constants (Kb) between carbonic anhydrase B (CAB, EC 4.2.1.1, from bovine erythrocytes) and charged benzenesulfonamides by affinity capillary electrophoresis (ACE) under conditions in which the migration time is affected by changes in electroosmotic flow and by nonspecific interactions accompanying changes in(More)
Local anesthetics are useful for reducing acute pain, but their short duration precludes them from use in solely managing postoperative pain. To prolong the duration of local anesthesia, we conjugated bupivacaine to native hyaluronan (HA) and divinyl sulfone cross-linked Hylan A (Hylan B particles) using a hydrolyzable linker incorporating an imide.(More)
Engineering proteins for selective tissue targeting can improve therapeutic efficacy and reduce undesired side effects. The relatively high dose of recombinant human acid α-glucosidase (rhGAA) required for enzyme replacement therapy of Pompe disease may be attributed to less than optimal muscle uptake via the cation-independent mannose 6-phosphate receptor(More)
Affinity capillary electrophoresis (ACE) provides a new approach to studying protein-ligand interactions. The basis for ACE is the change in the electrophoretic mobility of the protein when it forms a complex with its ligand. This binding interaction can be quantified directly for charged ligands or indirectly for neutral ligands in competition with a(More)
This paper describes a procedure, based on competitive binding, for identifying tight-binding ligand(l)Ig a receptor in mixtures of equimolar ligand candidates using affinity capillary electrophoresis (ACE). Vancomycin and a small library of 32 peptides were used as a model syatem to illushate the procedureThis procedure should be applicable to peptide(More)
The cation-independent mannose 6-phosphate receptor (CI-MPR) plays a critical role in intracellular transport of lysosomal enzymes as well as the uptake of recombinant proteins. To define the minimal glycan structure determinants necessary for receptor binding and cellular uptake, we synthesized a series of glycans containing mono-, di-, tri-, tetra-, and(More)
This work evaluates the use of a plug of enzyme, migrating in an electrophoresis capillary under nondenaturing conditiong, to convert substrate (which may be injected onto the capillary as a separate plug or included in the electrophoresis buffer) to product. This concept is demonstrated using two systems: the irreversible oxidation of glucose-G-phosphate(More)
We proposed the use of opioid drug bound covalently to hyaluronan (HA) via ester linkages as a method to prolong drug delivery and to possibly increase the quality of perioperative pain management. The in vitro release profile of morphine conjugated to HA (1.3 million MW) was studied. The influence of parameters such as conjugation site and steric(More)
Treatment of organotrineopentoxyphosphonium trifluoromethanesulfonates with base and organic peroxides results in carbon to phosphorus (C-P) bond cleavage. The products of the homolytic hydrolysis are hydrocarbons and trineopentyl phosphate. Reaction of the organophosphonium ions with only base leads to oxygen to phosphorus (0-P) bond cleavage with a(More)
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