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  • Luis G Brieba, Brandt F Eichman, Robert J. Kokoska, Sylvie Doublié, Tom A Kunkel, Tom E. Ellenberger
  • Physics, Medicine, Biology
  • The EMBO journal
  • 2004 (First Publication: 1 September 2004)
  • Accurate DNA replication involves polymerases with high nucleotide selectivity and proofreading activity. We show here why both fidelity mechanisms fail when normally accurate T7 DNA polymeraseExpand
  • Fernanda Cornejo-Granados, Alonso A Lopez-Zavala, +7 authors Adrián Ochoa-Leyva
  • Biology, Medicine
  • Scientific Reports
  • 2017 (First Publication: 18 September 2017)
  • Crustaceans form the second largest subphylum on Earth, which includes Litopeneaus vannamei (Pacific whiteleg shrimp), one of the most cultured shrimp worldwide. Despite efforts to study the shrimpExpand
  • Luis G Brieba, Robert J. Kokoska, Katarzyna Bębenek, T. A. Kunkel, Tom E. Ellenberger
  • Biology, Medicine
  • Structure
  • 2005 (First Publication: 1 November 2005)
  • 8-oxo-7,8-dihydroguanosine (8oG) is a highly mutagenic DNA lesion that stably pairs with adenosine, forming 8oG(syn).dA(anti) Hoogsteen base pairs. DNA polymerases show different propensities toExpand
  • J Huang, Luis G Brieba, Rui Sousa
  • Chemistry, Medicine
  • Biochemistry
  • 2000 (First Publication: 1 September 2000)
  • We have characterized the misincorporation properties of wild-type (wt) T7 RNAP and of 45 T7RNAP point mutants. The wt enzyme selects strongly against incorporation of an incorrect nucleotide. FromExpand
  • Noushin Ghaffari, Alejandro Sanchez-Flores, +14 authors Michael F. Criscitiello
  • Biology, Medicine
  • Scientific reports
  • 2014 (First Publication: 25 November 2014)
  • We present a new transcriptome assembly of the Pacific whiteleg shrimp (Litopenaeus vannamei), the species most farmed for human consumption. Its functional annotation, a substantial improvement overExpand
  • Vijaya Gopal, Luis G Brieba, Richard Guajardo, William McAllister, Rui Sousa
  • Biology, Medicine
  • Journal of molecular biology
  • 1999 (First Publication: 1 July 1999)
  • We have characterized the roles of the phage T7 RNA polymerase (RNAP) thumb subdomain and the RNA binding activity of the N-terminal domain in elongation complex (EC) stability by evaluating howExpand
  • Teresa Mejía-Castillo, María Eugenia Hidalgo-Lara, Luis G Brieba, Jaime Ortega-López
  • Medicine, Biology
  • Biotechnology Letters
  • 2007 (First Publication: 1 April 2008)
  • A cellulose-binding protein of 105 kDa (CBP105) from Cellulomonas flavigena was purified and its gene was cloned. CBP105 is a processive endoglucanase with maximum activity on carboxymethyl celluloseExpand