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Estrogen controls key cellular functions of responsive cells including the ability to survive, replicate, communicate and adapt to the extracellular milieu. Changes in the expression of 8400 genes were monitored here by cDNA microarray analysis during the first 32 h of human breast cancer (BC) ZR-75.1 cell stimulation with a mitogenic dose of(More)
BACKGROUND Estrogen receptors alpha (ERα) and beta (ERβ) are transcription factors (TFs) that mediate estrogen signaling and define the hormone-responsive phenotype of breast cancer (BC). The two receptors can be found co-expressed and play specific, often opposite, roles, with ERβ being able to modulate the effects of ERα on gene transcription and cell(More)
Estrogens control cell growth and viability in target cells via an interplay of genomic and extragenomic pathways not yet elucidated. Here, we show evidence that cell proliferation and survival are differentially regulated by estrogen in rat pituitary tumor PR1 cells. Pico- to femtomolar concentrations of 17beta-estradiol (E2) are sufficient to foster PR1(More)
BACKGROUND Microarray experiments enable simultaneous measurement of the expression levels of virtually all transcripts present in cells, thereby providing a 'molecular picture' of the cell state. On the other hand, the genomic responses to a pharmacological or hormonal stimulus are dynamic molecular processes, where time influences gene activity and(More)
The DNA microarray technique makes it possible to analyze the expression patterns of tens of thousands genes in a short time [1, 2]. The wide use of this technique and the rapidly improving different technologies available by several commercial and academic providers has led to the publication of thousands of results, extremely heterogeneous with respect to(More)
The DNA microarray technique is a powerful method that provides the opportunity to analyze the expression patterns of tens of thousands genes in a short time [1, 2]. Presently, several commercial and academic providers offer printed DNA microarrays, also known as chips, prepared according to a variety of technologies. In some cases, amplified cDNAs are(More)
2 Estrogens control cell growth and viability in target cells via a interplay of genomic and extra-genomic pathways not yet elucidated. Here we show evidence that cell proliferation and survival are differentially regulated by estrogen in rat pituitary tumour PR1 cells. Pico to femtomolar concentrations of 17β-estradiol (E2) are sufficient to foster PR1(More)
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