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Clinical application of induced pluripotent stem cells (iPSCs) is limited by the low efficiency of iPSC derivation and the fact that most protocols modify the genome to effect cellular reprogramming. Moreover, safe and effective means of directing the fate of patient-specific iPSCs toward clinically useful cell types are lacking. Here we describe a simple,(More)
It has been proposed that cumulative somatic mutations contribute to the aging process by disrupting the transcriptional networks that regulate cell structure and function. Experimental support for this model emerged from a recent study of cardiomyocytes that showed a dramatic increase in the transcriptional heterogeneity of these long-lived postmitotic(More)
We report here a systematic, quantitative population analysis of transcription factor expression within developmental progenitors, made possible by a microfluidic chip-based "digital RT-PCR" assay that can count template molecules in cDNA samples prepared from single cells. In a survey encompassing five classes of early hematopoietic precursor, we found(More)
The therapeutic promise of induced pluripotent stem cells (iPSCs) has spurred efforts to circumvent genome alteration when reprogramming somatic cells to pluripotency. Approaches based on episomal DNA, Sendai virus, and messenger RNA (mRNA) can generate "footprint-free" iPSCs with efficiencies equaling or surpassing those attained with integrating viral(More)
Electrophoretic data from both sodium dodecyl sulfate-polyacrylamide gels (SDS-PAGE) and acid-urea gels reveal a protein in purified murine mammary tumor virus (MuMTV) which co-migrates with purified chick skeletal muscle actin. 125I-labeling of intact and disrupted virus preparations shows that the actin-like protein is not artifactually adsorbed to the(More)
1 The Digital PCR Assay DNA quantitation in the Digital Array is based on the partitioning of a TaqMan PCR reaction into an array of 765 compartments or wells. If there are only a few template molecules in the sample, most of the wells capture either one or no molecules, and the number of positive wells at the PCR end-point gives a count of the molecules in(More)
  • Chrysa Samara, Postdoctoral Fellow, Luigi Warren, Peng Shi, Chris Rohde, Cody Gilleland +12 others
  • 2011
Overview of group: Our lab is developing high-throughput high-content technologies for investigating the complex development, function, reprogramming, degeneration and regeneration of the nervous system. We employ a variety of techniques including micromanipulation, microfluidics, ultrafast optics, advanced microscopy, quantum physics, genetics, and(More)
Adrenergic neuron blockade was induced by guanethidine in the pithed rat preparation. The pressor response to spinal electric stimulation was inhibited. The guanethidine blockade was reversed or prevented not only by tricyclic antidepressants but also by tripelennamine (an H1 antihistamine), tranylcypromine (a monoamine oxidase inhibitor) and viloxazine (a(More)
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