Ludovic G. Vincent

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Stem cells regulate their fate by binding to, and contracting against, the extracellular matrix. Recently, it has been proposed that in addition to matrix stiffness and ligand type, the degree of coupling of fibrous protein to the surface of the underlying substrate, that is, tethering and matrix porosity, also regulates stem cell differentiation. By(More)
Though reduced serum or myoblast co-culture alone can differentiate adipose-derived stem cells (ASCs) into mesenchymal lineages, efficiency is usually not sufficient to restore function in vivo. Often when injected into fibrotic muscle, their differentiation may be misdirected by the now stiffened tissue. Here ASCs are shown to not just simply reflect the(More)
A number of subjective and objective methods have been proposed, and are in use, to determine the roundness of sedimentary rock particles. Roundness, which is one of three properties describing the shape of a particle, is a measure of the extent to which the corners and edges of a particle have been worn away. The objective roundness measures are mainly(More)
Human mesenchymal stem cell (hMSC) proliferation, migration, and differentiation have all been linked to extracellular matrix stiffness, yet the signaling pathway(s) that are necessary for mechanotransduction remain unproven. Vinculin has been implicated as a mechanosensor in vitro, but here we demonstrate its ability to also regulate stem cell behavior,(More)
Mesenchymal stem cells (MSCs) respond to the elasticity of their environment, which varies between and within tissues. Stiffness gradients within tissues can result from pathological conditions, but also occur through normal variation, such as in muscle. MSC migration can be directed by shallow stiffness gradients before differentiating. Gradients with fine(More)
Cell patterning is typically accomplished by selectively depositing proteins for cell adhesion only on patterned regions; however in tissues, cells are also influenced by mechanical stimuli, which can also result in patterned arrangements of cells. We developed a mechanically-patterned hydrogel to observe and compare it to extracellular matrix (ECM) ligand(More)
Human mesenchymal stem cells (hMSCs) receive differentiation cues from a number of stimuli, including extracellular matrix (ECM) stiffness. The pathways used to sense stiffness and other physical cues are just now being understood and include proteins within focal adhesions. To rapidly advance the pace of discovery for novel mechanosensitive proteins, we(More)
There is great interest in point-of-care antibody testing for the diagnosis of infectious and autoimmune diseases. As a first step in the development of self-contained and miniaturized devices for highly quantitative antibody detection, we demonstrate the application of Luciferase Immunoprecipitation Systems (LIPS) technology in a microfluidic format.(More)
In their native environment adult stem cells are surrounded by a matrix, which provides biochemical signals and a structure for physical cell–matrix interactions to occur. In fact, cells actively deform and remodel their extracellular matrix (ECM), probe its rigidity and porosity, and undergo lineage-specific differentiation in part by integrating various(More)
Orthodontists mainly use stainless austenitic wires with a wide range of ultimate tensile strength. To characterize the dynamical behavior of these wires, the authors have perfected machines to test materials with their original surface roughness without any prior machining of surfaces. Fatigue limits are discussed through mechanical and structural(More)