Learn More
To explain the distinctive pharmacological profiles observed for adrenergic stimulation of cell proliferation (beta1) and cell differentiation (beta3), the adrenergic control of cAMP accumulation was investigated during brown adipocyte development. In preadipocytes, norepinephrine (NE) increased cAMP levels but the beta3-agonists BRL-37344 and CGP-12177 did(More)
The ligand-binding characteristics (B(max) and K(D)) of alpha(1)- and beta(1)/beta(2)-adrenoceptors were investigated in membranes prepared from brown adipose tissue (BAT) of warm-acclimated, cold-acclimated, hibernating and arousing ground squirrels (Spermophillus undulatus) and hamsters (Mesocricetus auratus) by specific binding of [(3)H]prazosin and(More)
A fraction with mol wt < 1 kDa was obtained from the brown fat of hibernating ground squirrels (Citellus undulatus) by means of delipidization, acid extraction, ultracentrifugation and ultrafiltration. This fraction suppressed the proliferation of mouse lymph node cells under standard mitogenic stimuli for T lymphocytes. In contrast, the fraction with mol(More)
Seasonal changes in the amount of DSIP-like material have been measured in extracts from the brain of Citellus suslicus. 2. Analysis of extracts from the brain of deeply sleeping ground squirrels shows that the level of enkephalin-like material reliably increases while that of ACTH-like material decreases. 3. It is concluded that the opiate brain activation(More)
Activities of mitochondrial electron transport chain enzymes NADH-CoQ oxidoreductase (complex I), cytochrome C-oxidase (complex IV), and citrate synthase were measured by spectrophotometry in m. quadriceps femoris homogenate from old rats receiving olive oil with the ration. Reduced activities of complexes I and IV in old animals were restored to the level(More)
Stimulation of DNA and protein synthesis in brown preadipocytes by 1 microM neokyotorphin in serum-containing media was comparable with the effect of 1 microM norepinephrine. In serum-free medium a decrease and a shift of the maximal effect to lower concentration of neokyotorphin were observed. Kyotorphin had no effect on cell proliferation in either(More)
One-week cold exposure of mice led to a 2-fold increase in the density of alpha1-adrenoceptors in brown adipose tissue. The density of alpha1-adrenoceptors returned to normal after adaptation to cold for 2 weeks. The reduced Ca2+ signaling in stem cells of brown fat activated via beta-adrenoceptors and cAMP was transformed into the Ca2+-system induced by(More)