Ludmila Dolmatova

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This paper clarifies the distribution and ecology of representatives of the genus Stuckenia (S. chakassiensis (Kaschina) Klinkova, S. pectinata (L.) Börner, S. macrocarpa (Dobroch.) Tzvelev, and S. vaginata (Turcz.) Holub) in brackish and saline lakes in eastern Russia. It is shown that S. chakassiensis grows in most Transbaikal lakes with a high salinity(More)
The possibility of using erythrocytic ganglioside diagnostic reagents (EGDR) for the detection of V. cholerae, E. coli and S. typhimurium enterotoxins in the passive hemagglutination (PHA) test has been shown. Museum strains and cultures isolated from patients with acute intestinal diseases were tested for the presence of enterotoxins. Cell-free extracts(More)
The possibility of obtaining biologically active cholerogen by extracting it from solid culture media used for the cultivation of vibrios is shown. V. cholerae, strain 569B, cultivated on solid media produced about 3 times more toxin per 1 ml of the culture medium than during the process of its cultivation with the use of liquid culture media. The(More)
In this work the possibility of using neuraminidase for increasing the content of ganglioside GM1 in the mixture of gangliosides used for the sensitization of erythrocytes has been studied. The study has revealed that the treatment of gangliosides with neuraminidase is sufficient for obtaining active hemosensitin; there is no need for the purification of(More)
A study was made to establish the possibility of using an immune adsorbent based on polyacrylamide gel with the concentration of T = 10% and C = 25% for the purification of cholerogen and antibodies to it. For the isolation of cholera toxin the gamma globulin fraction of antitoxic serum was incorporated into the adsorbent; for obtaining antibodies an(More)
The studies described in this work have indicated that cholera enterotoxin and its components (cholerogenoid and subunit B) can be detected in amounts of 0.25, 0.28 and 0.6 microgram of protein per 1 ml, respectively, by means of erythrocytes sensitized with gangliozide-containing complex. The conditions for erythrocyte sensitization have been established.(More)
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