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Chapter 2 Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology
TLDR
This chapter reviews the uses of one type, cell lines, in fish environmental toxicology and describes the nature of animal cell cultures, and explains the advantages ofAnimal cell cultures and toxicology applications and ecotoxicology applications of fish cell lines. Expand
Health of lake whitefish (Coregonus clupeaformis) with elevated tissue levels of environmental contaminants.
TLDR
The presence of liver neoplasms, gonadal lesions, and a decreased condition factor in lake whitefish from the St. Lawrence River may be etiologically related to elevated tissue concentrations of toxic chemical contaminants. Expand
The Use of Fish‐Derived Cell Lines for Investigation of Environmental Contaminants
TLDR
This unit describes protocols for growing salmonid cell lines and using them in in vitro toxicology studies, essential tools for investigating environmental toxicity at the cellular level. Expand
Development of a rainbow trout intestinal epithelial cell line and its response to lipopolysaccharide
TLDR
Cell viability declined in a LPS dose-dependent manner, allowing calculation of the concentration causing a 50% decline in viability (EC50), and glutamine and possibly AP protect against LPS cytotoxicity. Expand
Establishment of long term cultures of neural stem cells from adult sea bass, Dicentrarchus labrax.
TLDR
Sea bass brain tissues were dissected, dispersed and cultured in Leibovitz's L-15 media supplemented with 10% fetal bovine serum indicating their viability and suitability as convenient models for neurophysiological or neurotoxicological studies. Expand
Cytotoxicity evaluation of silica nanoparticles using fish cell lines
TLDR
Fish cell lines could be valuable for screening emerging contaminants in aquatic environments including NPs through rapid high-throughput cytotoxicity bioassays, in overall agreement with similar human and mouse cell studies reported to date. Expand
Initiation of a zebrafish blastula cell line on rainbow trout stromal cells and subsequent development under feeder-free conditions into a cell line, ZEB2J.
TLDR
A continuous cell line, ZEB2, was developed from zebrafish blastula-stage embryos expressing enhanced green fluorescent protein (GFP), which was heteroploid, had detectable telomerase activity, and was adherent. Expand
Developing a list of reference chemicals for testing alternatives to whole fish toxicity tests.
TLDR
The derivation of a list of 60 reference chemicals was detailed, selected as a prerequisite to gather mechanistic information on the performance of alternative testing systems, namely vertebrate cell lines and fish embryos, in comparison to the fish acute lethality test. Expand
Use of Tetrahymena thermophila To Study the Role of Protozoa in Inactivation of Viruses in Water
TLDR
The results suggest the active removal of T4 bacteriophage from fluid by macropinocytosis, followed by digestion in food vacuoles may be a mechanism occurring in natural waters and sewage treatment, and the methods described here could be used to study the factors influencing inactivation and possibly water quality. Expand
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