Learn More
The human papillomaviruses (HPV) are associated specifically with epithelial lesions, ranging from benign warts to invasive carcinoma. The virus encodes three late proteins, which are produced only in terminally differentiating keratinocytes, two of which are structural components of the virion. The third, E1-E4, is derived primarily from the E4 open(More)
Colicins are a diverse family of large antibacterial protein toxins, secreted by and active against Escherichia coli and must cross their target cell's outer membrane barrier to kill. To achieve this, most colicins require an abundant porin (e.g. OmpF) plus a low-copy-number, high-affinity, outer membrane protein receptor (e.g. BtuB). Recently, genetic(More)
We recently reported the expression of human papillomavirus type 18 (HPV-18) E6 protein in bacteria and the production of anti-E6 polyclonal antibodies. This work has now been extended with the production of a panel of monoclonal antibodies against the HPV-18 E6 protein. These antibodies demonstrate that there is little antigenic conservation in the E6(More)
The p53 is a nuclear protein that is associated with normal cellular proliferation and can cooperate with Ha-ras in causing cellular transformation in vitro. Lineage association is known to exist between p53 expression and normal lymphopoiesis, but not myelopoiesis. We studied the expression of p53 using chronic myelogenous leukemia (CML) cell lines,(More)
The human papillomavirus type 16 (HPV-16) E7 gene cooperates with an activated ras oncogene to transform primary rodent cells and is important in the immortalization of cervical keratinocytes. We have generated a series of point mutations within the E7 gene and show that mutation of residues serine 31 and serine 71 affect the phosphorylation of the E7(More)
Human papillomavirus (HPV) types 6 and 11 are usually found in benign genital lesions and laryngeal papillomas. However, the occasional occurrence of their DNAs in carcinomas of the genital tract and larynx suggests that they have some tumorigenic activity. In this paper, we have examined the cotransforming and transactivation activities of the E7 genes(More)
We have developed an alternative method for the assay of DNA binding activity of a known DNA binding protein. In contrast to standard gel retardation assays, this method is non-radioactive, quantitative and yields results within hours of commencement of the assay. Using an enzyme-linked immunosorbent assay (ELISA), the DNA binding activity of protein(More)