Lucia Becucci

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The intrinsic pKa values of the phosphate groups of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) and of the phosphate and carboxyl groups of phosphatidylserine (PS) in self-organized monolayers deposited on a hanging mercury drop electrode were determined by a novel procedure based on measurements of the differential capacity C of this(More)
A procedure based on surface plasmon resonance (SPR) is proposed to monitor the lateral mobility of lipid molecules in solid-supported bilayer lipid membranes (ssBLMs), an essential prerequisite for the formation of important microdomains called lipid rafts (LRs). The procedure relies on the marked tendency of the ganglioside GM1 to be recruited by LRs and(More)
Upon incorporating from 0.5 to 2 mol% ubiquinone-10 (UQ) in a self-assembled monolayer of dioleoylphosphatidylcholine (DOPC) supported by mercury, the kinetics of UQ reduction to ubiquinol-10 (UQH2) as well as that of UQH2 oxidation to UQ were investigated in borate buffers over the pH range from 8 to 9.5 by cyclic voltammetry. A general kinetic approach(More)
The nature and magnitude of the surface dipole potential chi at a membrane/water interface still remain open to discussion. By combining measurements of differential capacity C and charge density sigma at the interface between self-assembled monolayers of phosphatidylserine and phosphatidic acid supported by mercury and aqueous electrolytes of different(More)
A biomimetic membrane consisting of a thiolipid monolayer tethered to a mercury electrode, with a dioleoylphosphatidylcholine (DOPC) monolayer on top of it, was fabricated. The thiolipid, referred to as DPOL, consisted of an octaethyleneoxy (OEO) chain terminated at one end with a lipoic acid residue and covalently linked at the other end to two phytanyl(More)
A novel tethered bilayer lipid micromembrane (tBLmicroM) was prepared and characterized. It consists of a mercury cap electrodeposited on a platinum microelectrode, about 20 microm in diameter. The micromembrane was prepared by tethering to the mercury cap a thiolipid monolayer and by then self-assembling a lipid monolayer on top of it. The thiolipid(More)
The effect of the incorporation of phosphorylated phospholamban (pPLN) and sarcolipin (SLN) in mercury-supported self-assembled lipid monolayers and in lipid bilayers tethered to mercury via a hydrophilic spacer was investigated by voltammetric techniques and electrochemical impedance spectroscopy. It was shown that pPLN and SLN do not permeabilize lipid(More)
Tethered bilayer lipid membranes (tBLMs) consist of a lipid bilayer interposed between an aqueous solution and a hydrophilic "spacer" anchored to a gold or mercury electrode. There is great potential for application of these biomimetic membranes for the elucidation of structure-function relationships of membrane peptides and proteins. A drawback in the use(More)
The channel-forming activity of the lipodepsipeptide syringopeptin 25A (SP25A) was investigated at a tethered bilayer lipid membrane (tBLM) with a dioleoylphosphatidylcholine distal leaflet, anchored to a mercury electrode through a hydrophilic tetraethyleneoxy spacer. SP25A was incorporated in the tBLM from different aqueous solutions by recording a series(More)