Luca De Stefano

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We describe an innovative experimental and computational approach to control the expression of a protein in a population of yeast cells. We designed a simple control algorithm to automatically regulate the administration of inducer molecules to the cells by comparing the actual protein expression level in the cell population with the desired expression(More)
Porous silicon samples have been reduced in nanometric particles by a well known industrial mechanical process, the ball grinding in a planetary mill; the process has been extended to crystalline silicon for comparison purposes. The silicon nanoparticles have been studied by X-ray diffraction, infrared spectroscopy, gas porosimetry and transmission electron(More)
A procedure for fabrication of photomasks on photographic films with minimum feature achievable of about 20 μm, which are particularly suitable for the fast prototyping of microfluidic devices, has been improved. We used a commercial photographic enlarger in reverse mode obtaining 10:1 reduction factor with error less than 1%. Masks have been characterized(More)
In this work, two procedures for fabrication of polymeric microneedles based on direct photolithography, without any etching or molding process, are reported. Polyethylene glycol (average molecular weight 250 Da), casted into a silicone vessel and exposed to ultraviolet light (365 nm) through a mask, cross-links when added by a commercial photocatalyzer. By(More)
The recent scandal of poly implant prostheses (PIP), which were found in some cases to be made of non-medical grade silicone (as reported by the European Scientific Committee on Emerging and Newly Identified Health Risks), had a great social impact. Thousands of patients asked for implant removal with significant costs for public health care systems. We(More)
Finite element method analysis was applied to the characterization of the biomolecular interactions taking place in a microfluidic assisted microarray. Numerical simulations have been used for the optimization of geometrical and physical parameters of the sensing device. Different configurations have been analyzed and general considerations have been(More)
Micro-ring resonators have been widely employed, in recent years, as wavelength filters, switches and frequency converters in optical communication circuits, but can also be successfully used as transducing elements in optical sensing and biosensing. Their operation is based on the optical coupling between a ring-shaped waveguide and one or more linear(More)