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The distribution, function and ontogeny of the mononuclear phagocyte system of the chicken were characterised using the monoclonal antibody (MAb) KUL01. KUL01 specifically recognises chicken monocytes, macrophages and interdigitating cells, as well as activated microglia cells. Its tissue distribution allowed to discriminate KUL01 from all earlier described(More)
The objective of this study was to determine whether there exists a critical period during rearing when restricted feeding of broiler breeder hens can be most beneficial on subsequent egg production. Broiler breeder pullets were subjected to combinations of either ad libitum or restricted feeding during three periods before sexual maturity. Body weight(More)
The production and characterization of monoclonal antibodies (mAbs) toward the chicken pituitary glycoproteins are described. MAbs were first subdivided on an immunocytochemical (ICC) basis: one category of mAbs reacts with both thyrotropic and gonadotropic cells whereas another stains exclusively gonadotropic cells. The hypothesis that the former are(More)
1. The effect of the crude protein content (200 and 150 g/kg) of isoenergetic diets on episodic growth hormone (GH) release and on heat production was investigated in male broiler chickens. 2. Decreasing the crude protein content of isoenergetic diets from 200 g/kg (HP diet) to 150 g/kg (LP diet) resulted in depressed body weight gain, impaired food(More)
In an attempt to raise monoclonal antibodies to chicken pituitary glycoprotein hormones, mice were immunized with the concanavalin A-adsorbed components of a hypophyseal extract. Fusions of these spleen cells with myeloma cells repeatedly yielded hybridoma lines secreting antibodies that recognized specifically the pituitary caudal acidophils, known as the(More)
The interrelationship between immunoreactive inhibin and FSH was measured in chicken embryos from Day 8 to Day 20 of the incubation period. Plasma inhibin was measured by a heterologous RIA and FSH by a recently developed homologous RIA. Male and female inhibin plasma concentrations demonstrated a similar time course: a sharp increase with a peak on Day 13(More)
1. The concentrations of growth hormone (GH), insulin-like growth factor I (IGF-I), thyroxine (T4), triiodothyronine (T3), reverse-triiodothyronine (rT3), triglycerides (Tri), free fatty acids (FFA) and glucose (Glu) were determined at 2, 4 and 6 weeks of age in blood plasma of male and female chickens of broiler lines selected for body weight (GL) or food(More)
Fasting has severe effects on thyroid metabolism in the chicken: plasma thyroxine (T4) concentrations increase, whereas 3',5,3-triiodothyronine (T3) concentrations decrease. In the present report we studied the effect of fasting at the level of: 1) the pituitary (plasma thyrotropin (TSH) concentrations; the sensitivity of thyrotrophs to(More)
Research in mammals has demonstrated a variety of regulatory effects of vasopressin and oxytocin on endocrine functions of the anterior pituitary gland. Less evidence is available regarding the hypophysiotropic action of arginine vasotocin (AVT) comprising vasopressic and oxytocic activities in birds. Some hypophysiotropic effects of AVT may result from its(More)
Although pro-opiomelanocortin (POMC) is a well-known hormone precursor in many species, molecular information about avian POMCs is still relatively scarce. In a former study (Berghman et al., [1998] Mol Cell Endocrinol. 142:119-130) the nucleotide and amino acid sequence of N-terminal POMC in the chicken were reported. To complete the nucleotide sequence of(More)