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OBJECTIVE To evaluate the in vivo antimicrobial activity of chlorhexidine (CHX) in saliva 7 h after its application using an epifluorescence microscopy technique. SUBJECTS AND METHODS Fifteen volunteers performed a single mouthrinse with sterile water (SM-water) and with 0.2% CHX (SM-0.2% CHX). Saliva samples were taken at 30 s and 1, 3, 5 and 7 h after(More)
OBJECTIVES To analyse the influence of intrinsic and extrinsic factors on the in vivo antimicrobial activity of a chlorhexidine (CHX) digluconate mouthwash on the salivary flora up to 7h after its application, using epifluorescence microscopy. METHODS Ten volunteers performed the following mouthwashes: 0.12% CHX (10ml/30s, 15ml/30s and 10ml/1min); 0.2%(More)
The in vivo antimicrobial activity of 0.12% and 0.2% chlorhexidine (CHX) on the salivary flora up to 7 h after its application, using epifluorescence microscopy with the SYTO 9/propidium iodide dual staining, was evaluated. Fifteen volunteers performed a single mouthrinse with sterile water (SM-water), a single mouthrinse with 0.12% CHX (0.12% SM-CHX) and a(More)
OBJECTIVES To evaluate the in vivo antimicrobial activity on the salivary flora of a single mouthrinse of chlorhexidine (CHX) digluconate, analysing the influence of its concentration (0.2% versus 0.12%). METHODS The study group was formed of 20 adult volunteers with a good oral health status. Non-stimulated saliva samples were collected under basal(More)
OBJECTIVE To evaluate the in situ antibacterial activity of a mouthrinse with 0.2% Chlorhexidine (M-0.2% CHX) on undisturbed de novo plaque-like biofilm (PL-biofilm) and on salivary flora up to 7 hours after its application. METHODS A special acrylic appliance was designed, with 3 inserted glass disks on each buccal side, allowing for PL-biofilm growth.(More)
BACKGROUND The aim of the present study is to assess in situ substantivity of a single mouthrinse with 0.2% chlorhexidine (CHX) on saliva and on undisturbed de novo plaque-like biofilm (PL-biofilm), differentiating between two times of application: 1) CHX mouthrinse in the morning; and 2) CHX mouthrinse at night. METHODS The study participants were 10(More)
Our aim was to assess wounds made by lasers (CO(2) and Er,Cr:YSGG) for their epithelial architectural changes and width of damage. We allocated 60 Sprague-Dawley(®) rats into groups: glossectomy by CO(2) laser at 3 different wattages (n=10 in each); glossectomy by Er,Cr:YSGG laser at two different emissions (n=10 in each), and a control group (n=10).(More)
PURPOSE Sinus membrane perforation is the most common intraoperative complication of maxillary sinus floor elevation (MSFE) procedures and frequently causes postoperative problems. Piezoelectric devices have been claimed to reduce the frequency of membrane perforations although no clear evidence supports this view. MATERIALS AND METHODS Ten surgeons with(More)
Aspiration of foreign bodies is a serious complication that may occur during the course of dental treatment. A case report of a 60-year-old man with recurrent pneumonia is presented. One year after the onset of his initial symptoms, a hard substance that made a complete mold of the bronchial tree at the inferior right lobe was extracted with a rigid(More)
OBJECTIVE To evaluate the in vivo antimicrobial activity of different forms of application of chlorhexidine digluconate (CHX). METHOD AND MATERIALS A group of 10 volunteers performed mouth rinsings with a 0.2% and 0.12% solution of CHX and applied CHX in the form of a 0.2% gel, a 0.2% and a 0.12% spray and a swab impregnated with a 0.2% solution. Samples(More)