Lubomír Kováčik

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The limited specimen tilting range that is typically available in electron tomography gives rise to a region in the Fourier space of the reconstructed object where experimental data are unavailable - the missing wedge. Since this region is sharply delimited from the area of available data, the reconstructed signal is typically hampered by convolution with(More)
Ribosomal genes are organized in clusters termed Nucleolus Organizer Regions (NORs). Essential components of the RNA polymerase I transcription machinery, including Upstream Binding Factor (UBF), can be detected on some NORs during mitosis; these NORs, termed competent, are believed to be transcriptionally active during interphase. In cultured mammalian(More)
Using titrations of acetylcholine (ACh) on guinea-pig ileum the authors have demonstrated that there is no significant statistical difference between the cholinergic activity of extracts of 150 mg of retinal tissue from pilocarpinised eyes (0.3 mug of ACh) and the activity of extracts from control eyes not subjected to pilocarpine (0.29 mug of ACh). The(More)
Lon is an essential, multitasking AAA(+) protease regulating many cellular processes in species across all kingdoms of life. Altered expression levels of the human mitochondrial Lon protease (hLon) are linked to serious diseases including myopathies, paraplegia, and cancer. Here, we present the first 3D structure of full-length hLon using cryo-electron(More)
Electron tomographic reconstructions suffer from a number of artefacts arising from effects accompanying the processes of acquisition of a set of tilted projections of the specimen in a transmission electron microscope and from its subsequent computational handling. The most pronounced artefacts usually come from imprecise projection alignment, distortion(More)
In contrast to the well defined mechanism of merocrine exocytosis, the mechanism of apocrine secretion, which was first described over 180 years ago, remains relatively uncharacterized. We identified apocrine secretory activity in the late prepupal salivary glands of Drosophila melanogaster just prior to the execution of programmed cell death (PCD). The(More)
The phenotypic characterization and genetic identification of cyanobacteria according to modern polyphasic approach are difficult, especially in the case of coccoid and simple trichal and fine morphotypes with restricted morphological features. Molecular evaluation is not always possible because of the difficult transfer of all the natural populations into(More)
The authors have compared the content of acetylcholine (ACh) of the two ciliary bodies (150 microgram of tissue) after the instillation of a stimulating dose of a 1% aqueous solution of physostigmine (10 applications of three drops at intervals of three minutes) into the conjunctival fornix of one eye. Kymographic measurements on guinea-pig ileum(More)