Luís Presley

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The detection of DNA polymorphisms by RFLP analysis is having a major impact on identity testing in forensic science. At present, this approach is the best effort a forensic scientist can make to exclude an individual who has been falsely associated with an evidentiary sample found at a crime scene. When an analysis fails to exclude a suspect as a potential(More)
The analysis of DNA restriction fragment length polymorphisms by Southern blot hybridization requires that sufficient quantities of high molecular weight genomic DNA be extracted from biological specimens. Prior to analysis, it is necessary to determine the quantity and quality of the extracted DNA. For many applications, it is also desirable to determine(More)
The polymerase chain reaction (PCR) was used to amplify the HLA DQ alpha gene using DNA recovered from evidentiary samples. Amplified HLA DQ alpha DNA was then typed using sequence-specific oligonucleotide probes. Slight modifications of previously published DNA extraction methods improved typing success of bloodstains and semen-containing material.(More)
Deoxyribonucleic acid (DNA) restriction fragment length polymorphism (RFLP) profile results were obtained from bloodstains and other body fluid stains subjected to mixture with other body fluids, environmental insults (sunlight and temperature), different substrates (cotton, nylon, blue denim, glass, aluminum, and wood), and contaminants (gasoline, bleach,(More)
  • Vanessa Migliorini, Ana Lucia Machadoa, +5 authors Sebastião Vicente Canevaroloc
  • 2007
Department of Dental Materials and Prosthodontics, Araraquara Dental School, São Paulo State University, Rua Humaitá, 1680, Centro, 14801-903 Araraquara, SP, Brazil Interdisciplinary Laboratory of Eletrochemistry and Ceramics, Department of Chemistry, São Carlos Federal University, Rodovia Washington Luís (SP – 310), Km 235, 13565-905 São Carlos, SP, Brazil(More)
PURPOSE Fungal contamination of contact lenses is a common occurrence. In this study, we tested the effectiveness of currently used soft contact lens care systems against Aspergillus fumigatus. METHODS We prepared and added 100 microL inoculums of 1 x 10(6) colony forming units/mL to each test solution. We exposed fungi to disinfectant for 4, 6, and 8(More)
The apparent stability of DNA in forensic samples has permitted the successful application of several techniques such as polymerase chain reaction (PCR)-based and restriction fragment length polymorphisms (RFLP) analysis to forensic cases. PCR-based typing of the HLA-DQ alpha region in forensic casework has been shown to be a valid and reliable technique.(More)
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