Lovro Ziberna

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Despite being reported to reduce the risk of cardiovascular diseases, little is known about acute direct effects of bilberry anthocyanins on whole mammalian heart under ischemia–reperfusion (I–R) conditions. Bilberry anthocyanins were prepared from the ripe bilberries and analyzed using HPLC–DAD. Their antioxidant activity was evaluated by measuring the(More)
Berry anthocyanins have pronounced health effects, even though they have a low bioavailability. The common mechanism underlying health protection is believed to relate to antioxidant activity. Berry extracts, chemically characterised for their phenolic content, were prepared from bilberries (Vaccinium myrtillusL.) and blueberries (Vaccinium corymbosumL.);(More)
Anthocyanins exert neuroprotection in various in vitro and in vivo experimental models. However, no details regarding their brain-related pharmacokinetics are so far available to support claims about their direct neuronal bioactivity as well as to design proper formulations of anthocyanin-based products. To gather this missing piece of knowledge, we(More)
Water-soluble polymeric 3-alkylpyridinum salts (poly-APS), isolated from the marine sponge Reniera sarai, are natural products with promising biomedical applications. However, their ability to form non-specific cell membrane pores raises safety issues. Therefore, the aim of the present study was to investigate the direct toxic effects of poly-APS on the(More)
Direct analysis of free bilirubin in human and animal blood serum samples is reported for the first time. A state-of-the-art system comprised of newly developed high-performance liquid chromatography (HPLC) on reverse-phase (RP) C18 support coupled with thermal lens spectrometric detection (TLS), based on excitation at λ=457.9 nm by an argon laser was used(More)
Methods Anthocyanins from the prepared bilberry extract were analyzed using the HPLC-DAD system and were expressed as a standard of cyanidine-3-glucoside (mg/L). Experiments were carried out on the isolated hearts from Wistar rats of both sexes according to the Langendorff method. Postischemic myocardial injuries during reperfusion were determined by(More)
We present the applicability of a new ultra-sensitive analytical method for the simultaneous determination of biliverdin and bilirubin in human serum. The method comprises isocratic reversed-phase (RP) C18 high-performance liquid chromatography (HPLC) and thermal lens spectrometric detection (TLS) based on excitation by a krypton laser emission line at(More)
BACKGROUND AND AIMS Anthocyanins, a sub-class of flavonoids, induce endothelium-dependent vasorelaxation, by activating endothelial nitric oxide synthase and consequently increasing production of the vasorelaxant agent nitric oxide. It is not yet clear if anthocyanin-induced vasorelaxation starts with their interaction with plasma membrane receptors in the(More)
Red wine polyphenols (RWP) induce nitric oxide (NO) and endothelium-derived hyperpolarization (EDH)-mediated coronary vasodilatation involving the redox-sensitive PI3-kinase/Akt-dependent pathway in the endothelium. However, there is a gap of knowledge in explaining how bioactive polyphenols initialize their signalling pathway in endothelial cells. Here, we(More)
Histamine is one of many neurotransmitters that have been implicated in cardiovascular functioning. Three subtypes of histamine receptors (H1, H2 and H3) have been identified in the different blood vessels of some animal species [1]. Accordingly, several studies showed in vitro differences of histamine vasomotor effects depending on the animal species,(More)