Louise van der Weyden

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Influenza viruses exploit host cell machinery to replicate, resulting in epidemics of respiratory illness. In turn, the host expresses antiviral restriction factors to defend against infection. To find host cell modifiers of influenza A H1N1 viral infection, we used a functional genomic screen and identified over 120 influenza A virus-dependency factors(More)
Embryonic stem cell technology revolutionized biology by providing a means to assess mammalian gene function in vivo. Although it is now routine to generate mice from embryonic stem cells, one of the principal methods used to create mutations, gene targeting, is a cumbersome process. Here we describe the indexing of 93,960 ready-made insertional targeting(More)
The recently cloned P2Y11 receptor is unique amongst P2Y receptors with its coupling to the adenylyl cyclase pathway. P2Y11 has previously been shown to be expressed in human acute promyelocytic leukemia (APL) HL-60 and NB4 cell lines, and both cell types elevate cyclic AMP (cAMP) levels upon stimulation with extracellular ATP. Acute erythroleukemic K562(More)
Insertional mutagens such as viruses and transposons are a useful tool for performing forward genetic screens in mice to discover cancer genes. These screens are most effective when performed using hundreds of mice; however, until recently, the cost-effective isolation and sequencing of insertion sites has been a major limitation to performing screens on(More)
Genomic instability is a key hallmark of cancer leading to tumour heterogeneity and therapeutic resistance. BRCA2 has a fundamental role in error-free DNA repair but also sustains genome integrity by promoting RAD51 nucleofilament formation at stalled replication forks. CDK2 phosphorylates BRCA2 (pS3291-BRCA2) to limit stabilizing contacts with polymerized(More)
Promoter methylation of the RAS-association domain family 1, isoform A gene (RASSF1A) is one of the most frequent events found in human tumours. In this study we set out to test the hypothesis that loss of Rassf1a can cooperate with inactivation of the adenomatous polyposis coli (Apc) gene to accelerate intestinal tumourigenesis using the Apc-Min (ApcMin/+)(More)
To the Editor: Kuroki et al. recently reported “the finished sequence of the chimpanzee Y chromosome”1. Their analyses included comparisons with previously reported DNA sequences from the human and chimpanzee Y chromosomes2,3. The article1 was based on the authors’ sequencing of 12.7 Mb from the PTB1 library, which represents the genome of one male(More)
Department of Haematology, University of Cambridge, and National Health Service Blood and Transplant, Cambridge Biomedical Campus, Cambridge, United Kingdom; Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, United Kingdom; Institut HospitaloUniversitaire L’Institut de Rythmologie et Modélisation Cardiaque, Hopital Xavier(More)
1Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom; 2Cambridge Institute for Medical Research, Addenbrooke’s Hospital, Cambridge, United Kingdom; 3The Babraham Institute, Babraham Research Campus, Cambridge, United Kingdom; 4The Institute of Cancer Research, Section of Haemato-Oncology, Surrey, United Kingdom; and 5St(More)