Loretta Pobłocka-Olech

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In the framework of the correlation between chemotaxonomy and chemical analysis studies, the chemical composition of the essential oils of four varieties of Thuja species cultivated in Poland - T. occidentalis 'globosa', T. occidentalis 'aurea', T. plicata and T. plicata 'gracialis' - were investigated by GC and GC-MS. Thirty-one compounds were identified(More)
INTRODUCTION Salicis Cortex, made from willow bark is a herbal remedy, which is standardised based on the content of salicin, a compound with analgesic and antiphlogistic properties. However, clinical trials suggest that other compounds also present in Salicis Cortex can contribute to the pharmacological effects. OBJECTIVE To characterise the composition(More)
The SPE-HPLC method was developed to determine an isosalipurposide (5) and its derivative, 6"-O-p-coumaroyl ester (6) in the bark of eight taxa (I-VIII) belonging to three species of the genus Salix and originating from a natural habitat or cultivated for pharmaceutical purposes. The chalcones were separated by HPLC under gradient elution with the(More)
The separation of nine phenol glycosides--salicin, salicortin, 2'-acetylsalicortin, populin, tremulacin, salidroside, triandrin, picein and helicin--by normal phase (NP), reversed phase (RP) HPLC techniques and a coupling of NP and RP monolithic silica columns was studied. Among the above nine compounds only five--salicin, populin, tremulacin, salidroside(More)
CONTEXT Keloids and hypertrophic scars are hyperproliferative skin disorders resulting in abnormal wound healing. In the prevention and treatment of keloids and hypertrophic scars, ointments containing heparin and onion extract are very popular. Their therapeutic effects, however, are still controversial and the mechanism of action is not fully understood.(More)
This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ,(More)
A SPE-HPTLC method was developed for the qualitative and quantitative analysis of procyanidin B(1) in willow barks. The chromatography was performed on HPTLC silica gel layer with the mobile phase chloroform-ethanol-formic acid (50:40:6 v/v/v), in the Automatic Developing Chamber-ADC 2. The methanol extracts from willow barks were purified by SPE method on(More)
Salidroside and rosavins (rosavin, rosarin, and rosin), biologically active compounds of Rhodiola rosea L., were analyzed in dried roots and rhizomes of the plants cultivated in Lithuania and naturally growing in Altai geographical region in Russian Federation. The quantitative analysis of the aqueous-ethanolic extracts prepared by percolation was performed(More)
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