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OBJECTIVE To establish a weight-marking method and evaluate Siraitia grosvenorii germplasms. METHOD The characters of 21 kinds of S. grosvenorii germplasms in the field and the lab were analyzed, seven important characters were selected to weight the marks. A general evaluation index was made and used to evaluate S. grosvenorii germplasms. RESULT The(More)
Total RNA was isolated from Siraitia grosvenorii fruit by the method of modified Trizol, according to S. grosvenorii fruit characteristics of rich phenols, polysaccharide, oil and proteins. The OD260/280, OD260/230, RNA integrity (RIN) and yield of the total RNA with this method were 2.01, 2.02, 9.50 and 260 mirog.g-1, respectively. The open reading frame(More)
To explore the growth and development and analyze the quality of the parthenocarpy fruit induced by exogenous hormones of Siraitia grosvenorii. the horizontal and vertical diameter, volume of the fruit were respectively measured by morphological and the content of endogenous hormones were determined by ELISA. The size and seed and content of mogrosides of(More)
A reliable and efficient hairy root induction system for the important medicinal plant Callerya speciosa was established by optimizing several parameters that affected the efficiency of Agrobacterium rhizogenes-mediated transformations. Four strains, A4, LBA9402, R1601 and C58C1, and four types of explants, hypocotyls, cotyledons, leaves and excised stems,(More)
OBJECTIVE To select the best formula for soilless cultivation of Nervilia fordii. METHODS Four different formulas of nutrient solution on growth,antioxidase activities and amino acid contents were investigated. RESULTS The concentration of 1/2 lettuce nutrient solution formula was the most propitious for the growth of Nervilia fordii, the antioxidase(More)
OBJECTIVE Establishing the rapid propagation for plant tissue culture of Smilax glabra in order to rationally protect and utilize the resource. METHODS The seed, shoot, leaf and rhizome of Similax glabra were cultured as explants. Different media and plant growth regulators at different concerntration were used. RESULTS The best medium for bud induction(More)
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