Lon J. Van Winkle

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Horse serum (HS) supported growth and differentiation of blastocysts as well as or better than fetal bovine serum (FBS) (p<0.01) as measured by a) the onset of trophoblastic outgrowth, b) the size of the resultant outgrowths, c) the size of nuclei in trophoblasts after their outgrowth and d) the size of the inner cell mass in outgrowths. Moreover,(More)
Stem cells are at the forefront of current regenerative and biomedical research. Thus, there exists an imperative and urgent need to understand the mechanisms that drive stem cell function in order to exploit their use as a therapeutic tool. Amino acids are potent inducers of signaling cascades that drive stem cell proliferation and differentiation. With a(More)
The stoichiometry of amino acid transport with co- or counter-substrates of a given system has been found to vary with the amino acid species. This phenomenon has been studied directly in only a few cases, however, by measuring the fluxes of the substrates simultaneously. More frequently, the apparent transport stoichiometries of single amino acid species(More)
Under identical in vitro labeling conditions, significantly lower amounts of radioactivity were found a) in proteins of early diapausing blastocysts (days 7–9 p.c.) vs normal, late blastocysts (day 5 p.c.) and b) in proteins of days 19–23 p.c. diapausing blastocysts from ovariectomized mothers treated with progesterone vs similar embryos from untreated(More)
Experimental delay of implantation was induced by ovariectomizing mice on the 4th day after mating. On the 2 days preceding sacrifice, which was on days 7–14 of pregnancy, 3 groups of mice received a s.c. injection of either progesterone (2.0 mg) in oil, oil, or no injection. Progesterone administration significantly reduced the number of blastocysts(More)
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