Lloyd E. King

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To assess the effects of transforming growth factor alpha (TGF-alpha) on mammalian skin in vivo, we have targeted its expression to the epidermis of transgenic mice using a vector based on the human K1 (HK1) gene. Neonatal mice expressing the HK1.TGF-alpha transgene were often smaller than normal littermates and had precocious eyelid opening and wrinkled,(More)
Elevated expression of matrix metalloproteinase-3 (MMP-3/stromely-sin-1) is associated with a variety of tumor types, although its in vivo functional role remains unclear. In human and murine squamous cell carcinoma (SCC), MMP-3 is expressed in the stromal compartment at all of the stages of tumor progression and is expressed by the malignant epithelial(More)
Recent data suggest that epidermal growth factor and epidermal growth factor receptors (EGF/EGF-R) are present and functional in neurons within the central nervous system. Previously, EGF was detected in developing and mature rat brain and cerebrospinal fluid. Also, EGF-R was documented in discrete locations in normal adult human brain, as well as in senile(More)
Alopecia areata (AA) is an autoimmune disease that attacks anagen hair follicles. Gene array in graft-induced C3H/HeJ mice revealed that genes involved in retinoic acid (RA) synthesis were increased, whereas RA degradation genes were decreased in AA compared with sham controls. This was confirmed by immunohistochemistry in biopsies from patients with AA and(More)
C57BL/6 mice develop dermatitis and scarring alopecia resembling human cicatricial alopecias (CAs), particularly the central centrifugal CA (CCCA) type. To evaluate the role of retinoids in CA, the expression of retinoid metabolism components were examined in these mice with mild, moderate, or severe CA compared with hair cycle-matched mice with no disease.(More)
There is increasing awareness of the role of mtDNA alterations in the development of cancer, as mtDNA point mutations are found at high frequency in a variety of human tumors. To determine the biological effects of mtDNA mutations in UV-induced skin tumors, hairless mice were irradiated to produce tumors, and the tumor mtDNAs were screened for(More)
Mice. C3H/HeJ +/+ (JR# 659) inbred mice were obtained from The Jackson Laboratory (Bar Harbor, ME). Mice were maintained in a humidity-, temperature-, and light cycle (12:12) controlled vivarium under specific pathogen-free conditions housed in double-pen polycarbonate cages (330 cm 2 floor area) at a maximum capacity of four mice per pen. Mice were allowed(More)
BACKGROUND There have been repeated initiatives to produce standard nosologies and terminologies for cutaneous disease, some dedicated to the domain and some part of bigger terminologies such as ICD-10. Recently, formally structured terminologies, ontologies, have been widely developed in many areas of biomedical research. Primarily, these address the aim(More)
Alopecia areata (AA), the second most common form of hair loss in humans, is characterized by waxing and waning focal hair loss. Alopecic foci can expand to encompass the entire head (alopecia totalis) or body (alopecia universalis, AU). This cell mediated autoimmune disease is characterized histologically by the " swarm of bees " , CD4 + and CD8 + T cells,(More)