• Publications
  • Influence
An efficient transformation method for Bacillus subtilis DB104
Bacillus subtilis strains are used for extracellular expression of enzymes (i.e., proteases, lipases, and cellulases) which are often engineered by directed evolution for industrial applications. B.Expand
  • 39
  • 3
A first continuous 4-aminoantipyrine (4-AAP)-based screening system for directed esterase evolution
Esterases hydrolyze ester bonds with an often high stereoselectivity as well as regioselectivity and are therefore industrially employed in the synthesis of pharmaceuticals, in food processing, andExpand
  • 15
  • 1
A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels.
A high throughput whole cell flow cytometer screening toolbox was developed and validated by identifying improved variants (1.3-7-fold) for three hydrolases (esterase, lipase, cellulase). TheExpand
  • 10
  • 1
  • PDF
Correction: A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels.
Correction for 'A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels' by Nina Lülsdorf et al., Chem. Commun., 2015, 51, 8679-8682.
  • 5
  • 1
  • PDF
Redirecting catalysis from proteolysis to perhydrolysis in subtilisin Carlsberg.
Enzyme promiscuity describes the ability of biocatalysts to catalyze conversions beyond their natural reactions. Enzyme engineering to promote side reactions is attractive for synthetic andExpand
  • 5
  • 1
Advances in protease engineering for laundry detergents.
Proteases are essential ingredients in modern laundry detergents. Over the past 30 years, subtilisin proteases employed in the laundry detergent industry have been engineered by directed evolutionExpand
  • 52
In vitro flow cytometry-based screening platform for cellulase engineering
Ultrahigh throughput screening (uHTS) plays an essential role in directed evolution for tailoring biocatalysts for industrial applications. Flow cytometry-based uHTS provides an efficient coverage ofExpand
  • 22
A fluorescent hydrogel-based flow cytometry high-throughput screening platform for hydrolytic enzymes.
Screening throughput is a key in directed evolution experiments and enzyme discovery. Here, we describe a high-throughput screening platform based on a coupled reaction of glucose oxidase and aExpand
  • 36
  • PDF
Rationalizing perhydrolase activity of aryl-esterase and subtilisin Carlsberg mutants by molecular dynamics simulations of the second tetrahedral intermediate state
The perhydrolysis reaction in hydrolases is an important example of catalytic promiscuity and has many potential industrial applications. The mechanisms of perhydrolase activity of a subtilisinExpand
  • 9
Fluorescent Assay for Directed Evolution of Perhydrolases
Directed evolution offers opportunities to improve promiscuous activities of hydrolases in rounds of diversity generation and high-throughput screening. In this article, we developed and validated aExpand
  • 16