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The CRISPR/Cas9 system is a powerful genome editing tool and has been widely used for biomedical research. However, many challenges, such as off-target effects and lack of easy solutions for multiplex targeting, are still limiting its applications. To overcome these challenges, we first developed a highly efficient doxycycline-inducible Cas9-EGFP vector.(More)
The complexity by which cells regulate gene and protein expression is multifaceted and intricate. Regulation of 3' untranslated region (UTR) processing of mRNA has been shown to play a critical role in development and disease. However, the process by which cells select alternative mRNA forms is not well understood. We discovered that the Saccharomyces(More)
Recently, Distribute Generation sources (DGs) connected to the distribution network has received increasing attention. The daily voltage control is one of the most important schemes in distribution networks, which can be affected by DGs. This paper presents a multi-objective Voltage optimizes control modeling, including objectives that are the total cost of(More)
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