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Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells
Measuring gene expression in individual cells is crucial for understanding the gene regulatory network controlling human embryonic development. Here we apply single-cell RNA sequencing (RNA-Seq)Expand
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The DNA methylation landscape of human early embryos
DNA methylation is a crucial element in the epigenetic regulation of mammalian embryonic development. However, its dynamic patterns have not been analysed at the genome scale in humanExpand
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The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells
Germ cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germExpand
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A single-cell RNA-seq survey of the developmental landscape of the human prefrontal cortex
The mammalian prefrontal cortex comprises a set of highly specialized brain areas containing billions of cells and serves as the centre of the highest-order cognitive functions, such as memory, cognitive ability, decision-making and social behaviour. Expand
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NRAS Mutations Are Rare in Colorectal Cancer
Activating mutations in members of the RAS oncogene family (KRAS, HRAS, and NRAS) have been found in a variety of human malignancies, suggesting a dominant role in carcinogenesis. In colon cancers,Expand
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Single-cell DNA methylome sequencing of human preimplantation embryos
DNA methylation is a crucial layer of epigenetic regulation during mammalian embryonic development1–3. Although the DNA methylome of early human embryos has been analyzed4–6, some of the key featuresExpand
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Sensitive sequencing method for KRAS mutation detection by Pyrosequencing.
Both benign and malignant tumors represent heterogenous tissue containing tumor cells and non-neoplastic mesenchymal and inflammatory cells. To detect a minority of mutant KRAS alleles among abundantExpand
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Whole-genome analysis of 5-hydroxymethylcytosine and 5-methylcytosine at base resolution in the human brain
Background5-methylcytosine (mC) can be oxidized by the tet methylcytosine dioxygenase (Tet) family of enzymes to 5-hydroxymethylcytosine (hmC), which is an intermediate of mC demethylation and mayExpand
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Spatial transcriptomic survey of human embryonic cerebral cortex by single-cell RNA-seq analysis
The cellular complexity of human brain development has been intensively investigated, although a regional characterization of the entire human cerebral cortex based on single-cell transcriptomeExpand
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Genome Analyses of Single Human Oocytes
Single-cell genome analyses of human oocytes are important for meiosis research and preimplantation genomic screening. However, the nonuniformity of single-cell whole-genome amplification hinderedExpand
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