Liyan Tang

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OBJECTIVE To construct Brucella vaccine strain M5-90 delta virB2 mutants. METHODS Suicide plasmid pGEM-7zf-delta virB2-sacB was constructed by traditional molecular biology technology. Through the method of homologous recombination, we screened Brucella gene deletion mutant by 100 mg/L ampicillin resistance screening and 5% sugar sensitivity screening(More)
Nonylphenol (NP) is an environmental chemical that affects apoptosis and male infertility. In our study, we found that a high concentration of NP could down-regulate the expression of microRNA-361-3p (miR-361-3p) in the murine GC-1 spermatogonia cell line and in vivo in murine spermatogonia. Additionally, one direct target of this miR, the 3' untranslated(More)
Brucella is Gram-negative intracellular bacterial pathogen that infects humans and animals and contributes to great economic losses in developing countries. Presently, live attenuated Brucella vaccines (Brucella melitensis M5-90) are the most effective means of brucellosis control and prevention in animals. However, these vaccines have several drawbacks,(More)
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