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A mist bioreactor using a disposable bag as culture chamber was used to propagate carrot embryogenic cells into rooted plantlets. The best operating configuration was akin to a vertical hanging garden using 50–90 μm nylon mesh for explant attachment. Cells spray inoculated into the reactor were 51.2 % viable. Misting cycle and aeration conditions were(More)
A mist bioreactor using a disposable bag as a culture chamber was used to propagate single nodal explants of Artemisia annua into rooted plantlets that were ready for soil using a single batch (one-step) culture. To vertically scale plant growth inside the mist reactor, poly-l-lysine (PLL)-coated 70 µm nylon mesh and solid polypropylene sheeting were used(More)
The medicinally important plant Artemisia annua was used as an example to study the effect of mist reactor conditions on shooting, rooting, and in vitro acclimatization. During all three stages, plants grown in the mist reactor yielded more shoot biomass than corresponding gelled medium controls. During shoot development, shooting percentage and shoot(More)
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