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Soil-borne cereal mosaic virus (SBCMV) was first reported affecting wheat crops in Italy in 1960 and has spread subsequently to many other European countries, including the UK. SBCMV causes a serious disease of wheat, reducing yield by up to 70%; growing resistant varieties represents the only economical means of control. Real-time RT-PCR and PCR assays(More)
A complete review of the records of plant virus, viroid, liberibacter and phytoplasma in New Zealand has found evidence for 220 viruses, seven viroids, two liberibacters and two phytoplasmas. Of these, 80 viruses, one viroid and two species of liberibacter have been reported as new to New Zealand since the last review in 2006. Ten viruses and two viroids,(More)
Two degenerate primer pairs were designed for the universal detection of members of the genus Torradovirus. Primer pair Torrado-1F/Torrado-1R was designed based on the RNA-dependent RNA polymerase region located in RNA1 and primer pair Torrado-2F/Torrado-2R based on a region overlapping the two first coat proteins Vp35 and Vp26 in RNA2. The primers were(More)
Targeted surveillance was done in 2004/2005 and 2007 to determine the presence of viruses in ornamental and vegetable Allium crops in New Zealand. Several samples were tested for regulated viruses and viruses that commonly infect New Zealand’s Allium crops using enzyme-linked immunosorbent assay and reverse transcription-polymerase chain reaction. Most(More)
A new ilarvirus was detected in wild radish in New Zealand. Numerous isometric particles (Ø 35 nm) were seen by electron microscopy. Partial sequences for RNA1, RNA2 and RNA3 were obtained. Based on sequence data, the virus belongs to the Ilarviruses subgroup 1, which members normally cause diseases of economic importance in their hosts, however it is(More)
A TaqMan-based real-time one-step RT-PCR assay was developed for the rapid detection of Tomato black ring virus (TBRV), a significant plant pathogen which infects a wide range of economically important crops. Primers and a probe were designed against existing genomic sequences to amplify a 72 bp fragment from RNA-2. The assay amplified all isolates of TBRV(More)
Loop-mediated isothermal amplification (LAMP) is a technique involving the use of four to six primers (two inner primers, two outer primers, and two loop primers) and the strand displacement activity of Bacillus subtilis-derived (Bst) DNA polymerase. The end result of strand displacement and loop formation and synthesis is the single-temperature(More)
A survey was carried out to confirm which viruses infecting Rosa spp. are present in New Zealand and to determine the extent of their distribution. Eighty nine rose samples were collected from different regions across New Zealand and from various sources as follows, private and public gardens, a commercial glasshouse and native bush. The samples were tested(More)
Pea mild mosaic virus (PMiMV) was reported from pea (Pisum sativum) in New Zealand in 1972. As this remains the only report of this virus worldwide, we aimed to try and clarify the status of PMiMV in New Zealand. In this study, nine species of comovirus were tested against antisera believed to have been raised to PMiMV and other comoviruses in 1972, and(More)
Detection and preventing entry of exotic viruses and viroids at the border is critical for protecting plant industries trade worldwide. Existing post entry quarantine screening protocols rely on time-consuming biological indicators and/or molecular assays that require knowledge of infecting viral pathogens. Plants have developed the ability to recognise and(More)