Lisa A. Stehno-Bittel

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The nucleus is surrounded by a double membrane separating it from the cytoplasm. The perinuclear space is continuous with endoplasmic reticulum, and the nuclear outer membrane shares many features with the reticular membrane. We now show that inositol 1,4,5-trisphosphate (InsP3) receptors associated with the nucleus release Ca2+ from isolated Xenopus laevis(More)
Intact, isolated nuclei and a nuclear membrane (ghost) preparation were used to study regulation of the movement of small molecules across the Xenopus laevis oocyte nuclear membrane. In contrast to models of the nuclear pore complex, which assume passive bidirectional diffusion of molecules less than 70 kilodaltons, diffusion of intermediate-sized molecules(More)
The nuclear pore complex (NPC) is essential for the transit of molecules between the cytoplasm and nucleoplasm of a cell and until recently was thought to allow intermediate-sized molecules (relative molecular mass of approximately 10,000) to diffuse freely across the nuclear envelope. However, the depletion of calcium from the nuclear envelope of Xenopus(More)
Barriers to the use of islet transplantation as a practical treatment for diabetes include the limited number of available donor pancreata. This project was designed to determine whether the size of the islet could influence the success rate of islet transplantations in rats. Islets from adult rats were divided into two groups containing small (diameter(More)
Patients with diabetes mellitus incur a higher incidence of fractures compared to healthy individuals. This suggests that the structural integrity of the skeletal system may be compromised. To examine the biomechanical consequences of diabetes, we studied the structural integrity of the femur and tibia of rats with streptozotocin-induced diabetes. The(More)
Chemokines play a key role in the regulation of central nervous system disease. CXCL10 over-expression has been observed in several neurodegenerative diseases, including multiple sclerosis, Alzheimer's disease and HIV-associated dementia. More recent studies by others and us have shown that CXCL10 elicits apoptosis in fetal neurons. The mechanism of(More)
Chemokine (C-C motif) ligand 2 (CCL2), also known as monocyte chemoattractant protein-1, plays a critical role in leukocyte recruitment and activation. In the present study, we identify an additional role for CCL2 that of neuroprotection against HIV-1 transactivator protein (Tat) toxicity in rat primary midbrain neurons. Furthermore, we report the(More)
At least 30 G protein-linked receptors stimulate phosphatidylinositol 4,5-bisphosphate phosphodiesterase (phospholipase C beta, PLC beta) through G protein subunits to release intracellular calcium from the endoplasmic reticulum (Clapham, D. E. (1995) Cell 80, 259-268). Although both G alpha and G beta gamma G protein subunits have been shown to activate(More)
One of the most exciting cell biology fields of study concerns the physiology and pathology of fat. The basic assumptions once held concerning the function of adipose tissue have been shown to be oversimplified or sometimes completely wrong. Fat does more than store excess energy; it is actually the largest endocrine organ in the body, and it may be one of(More)
INTRODUCTION/PURPOSE Exercise is an effective nonpharmacological treatment in the prevention of mortality and morbidity due to cardiovascular disease in Type I diabetes. This study sought to explore the effects of endurance exercise on the ultrastructural changes seen in diabetic cardiomyopathy. METHODS Seven-week-old rats were divided into three groups(More)