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Molecular cloning of a laccase gene from Ganoderma lucidum and heterologous expression in Pichia pastoris
A genomic laccase gene and cDNA were cloned from the white‐rot fungi Ganoderma lucidum TR6 and both were expressed in the Pichia pastoris GS115, finding the optimal pH and temperature were specific at pH 3.5 and special range from 60 to 90 °C. Expand
Production, purification and characterization of a thermostable laccase from a tropical white-rot fungus
A thermostable laccase was isolated from a tropical white-rot fungus Polyporus sp. which produced as high as 69,738 units of laccase l−1 in an optimized medium containing 20 g of malt extract l−1, 2Expand
Non-volatile components of several novel species of edible fungi in China
Results showed that these four species of newly commercial edible mushrooms in China were distinctly different in non-volatile components. Expand
Highly efficient Agrobacterium-mediated transformation of Volvariella volvacea.
Agrobacterium tumefaciens-mediated transformation (ATMT) was successfully applied to the edible straw mushroom, Volvariella volvacea, and stable integration of the afp gene with 1-4 copy numbers was confirmed in all 10 randomly selected transgenic events by Southern blot analysis. Expand
A novel process for obtaining pinosylvin using combinatorial bioengineering in Escherichia coli
Three bioengineering strategies were described to the development of a simple and economical process for pinosylvin biosynthesis in Escherichia coli and clustered regularly interspaced short palindromic repeats interference was explored to inactivate malonyl-CoA consumption pathway to increase its availability. Expand
Expression of multi-functional cellulase gene mfc in Coprinus cinereus under control of different basidiomycete promoters.
Multi-functional cellulase gene mfc was expressed in Coprinus cinereus under naturally non-inductive conditions using three heterologous promoters using promoter sequences from the Lentinula edodesgpd gene, the Flammulina velutipes gpd gene and the Volvariella volvaceagpd gene. Expand
Cloning and characterization of squalene synthase gene from Poria cocos and its up-regulation by methyl jasmonate
A phylogenetic analysis revealed that P. cocos SQS belonged to the fungus group, and was more closely related to the SQS of Ganoderma lucidum than other fungi. Expand
Efficient transformation and expression of gfp gene in the edible mushroom Pleurotus nebrodensis
This is the first report that a “reporter” gene, green fluorescent protein gene can be successfully stably expressed in this Pleurotus species. Expand
Increased resveratrol production in wines using engineered wine strains Saccharomyces cerevisiae EC1118 and relaxed antibiotic or auxotrophic selection
Resveratrol content of the wine fermented with engineered strains was twice higher than that of the control, indicating that the engineered strains could increase the production of resveratol during wine fermentation. Expand
Biosynthesis of Resveratrol in Blastospore of the Macrofungus Tremella fuciformis
This study is the first to report about the transformation and expression of resveratrol biosynthetic genes in bTf, a significant step toward obtaining resver atrol-producing T. fuciformis strains, which not only satisfy the demand of res veratrol market, but also expand the category of functional food. Expand