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The peel yellowing is an important pigment physiological process of green fruit ripening, which mainly results from chlorophyll degradation in the fruit peel. In this work, two typical cultivars with different ripening speed, a slow ripening pear 'Emerald' (Pyrus bretschneideri Rehd. cv. Emerald) and a fast ripening 'Jingbai' (Pyrus ussuriensis Maxim. cv.(More)
Gingseng is commonly used in traditional Chinese medicine community for the treatment of depression-like disorders. Ginsenosides is considered to be the major active components of ginseng. Previous studies have demonstrated that ginsenosides produced antidepressant-like action in various mouse models of behavioral despair. The present study aimed to examine(More)
With the development of molecular quantitative genetics, particularly, genetic linkage map construction, quantitative trait loci mapping or genes fine mapping and association analysis etc., more and more PCR products separated in polyacrylamide gels need to be silver-stained. However, conventional silver-staining procedures are complicated and(More)
BACKGROUND Ripening affects the quality and nutritional contents of fleshy fruits and is a crucial process of fruit development. Although several studies have suggested that ubiquitin-conjugating enzyme (E2s or UBC enzymes) are involved in the regulation of fruit ripening, little is known about the function of E2s in papaya (Carica papaya). (More)
Reverse transcription quantitative PCR (RT-qPCR) as the accurate and sensitive method is use for gene expression analysis, but the veracity and reliability result depends on whether select appropriate reference gene or not. To date, several reliable reference gene validations have been reported in fruits trees, but none have been done on preharvest and(More)
1-methylcyclopropene (1-MCP) has been shown to delay fruit ripening and senescence during storage. In this study, we investigated the effects of 1.0 μL·L−1 1-MCP on the biochemical changes and cellular ultra-structure of the core tissue in ‘Yali’ pear (Pyrus bretschneideri Rehd.) fruits stored at 25 ± 2°C. Our results showed that 1-MCP treatment(More)
The common Chinese cuttlefish (Sepiella japonica) has been considered one of the most economically important marine Cephalopod species in East Asia and seed breeding technology has been established for massive aquaculture and stock enhancement. In the present study, we used Illumina HiSeq2000 to sequence, assemble and annotate the transcriptome of the ovary(More)
‘Jingbaili’ pear (Pyrus ussuriensis Maxim.) fruit shows rapid softening, yellowing and short shelf-life at ambient temperature storage. In this study, ‘Jingbaili’ pear fruits were treated with 1.0 μL·L−1 1-methylcyclopropene (1-MCP) for 24 hours at 25 ± 2°C and then stored at 0°C. After 60 and 120 days of cold storage at 0°C, pear fruits were removed and(More)
In this paper, large area and deep sub-wavelength interference patterns are realized experimentally by using odd surface plasmon modes in the metal/insulator/metal structure. Theoretical investigation shows that the odd modes possesses much higher transversal wave vector and great inhibition of tangential electric field components, facilitating surface(More)