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The oriental river prawn, Macrobrachium nipponense, is an important crustacean species in aquaculture. However, early gonad maturity is a ubiquitous problem which devalues the product quality. While husbandry and nutritional management have achieved little success in tackling this issue, a molecular approach may discover the genes involved in reproduction(More)
The toxicity of ammonia to Eriocheir sinensis juveniles was determined. The 24 h-, 48 h-, 72 h-, 96 h-LC(50) values of total ammonia (TAN) were 251.68, 217.61, 156.05, and 119.67 mg L(-1), respectively. Following these results, crabs were then exposed for a 2-day period to 20, 40, 60 and 80 mg L(-1) TAN and sampled at 3, 6, 24 and 48 h for changes in(More)
Natural selection endows animals with the abilities to store lipid when food is abundant and to synthesize lipid when it is limited. However, the relevant adaptive strategy of lipid metabolism has not been clearly elucidated in fish. This study examined the systemic metabolic strategies of Nile tilapia to maintain lipid homeostasis when fed with low- or(More)
A two-stage study was carried out to test the response of juvenile darkbarbel catfish Pelteobagrus vachelli to ammonia stress, dietary lipid and bacterial challenge. At stage 1, the catfish (0.99 ± 0.01 g) fed a commercial diet were exposed to 0.01 and 5.70 mg L(-1) total ammonia nitrogen in nine replicates for 14 days. At stage 2, all fish previously(More)
Chemokines represent a superfamily of chemotactic cytokines involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci. We cloned and sequenced the cDNA of a CXC chemokine that is most similar to CXCL10 from channel catfish and blue catfish. Sequence analysis of PCR amplicons from a single F1 hybrid catfish(More)
Chemokines are important mediators for innate immunity involved in recruitment, activation and adhesion of a variety of leukocyte types to inflammatory foci. While almost all chemokines have been identified from mammals, only a handful of fish chemokines have been identified. Here we report molecular cloning, sequence analysis, and expression of a channel(More)
The lipopolysaccharide and beta-1, 3-glucan binding protein (LGBP) is a pattern recognition protein which is fundamental for the innate immune response of crustaceans. A partial cDNA produced by the random sequencing of cDNA clones from a haemocyte cDNA library of Eriocheir sinensis showed similarity to the LGBP gene of the Chinese shrimp (Fenneropenaeus(More)
Heat shock protein 90 (HSP90) is not only involved in environmental stress but also plays roles in the ovary development in some vertebrates. To understand its role in crustacean, we examined the HSP90 cDNA for the first time in the ovary and hepatopancreas of the oriental river prawn, Macrobrachium nipponense and designated this protein as MnHSP90 in this(More)
The gustavus gene is required for localizing pole plasm and specifying germ cells. Research on gustavus gene expression will advance our understanding of the biological function of gustavus in animals. A cDNA encoding gustavus protein was identified and termed MnGus in the oriental river prawn Macrobrachium nipponense. Bioinformatic analyses showed that(More)
Peroxisome proliferator activated receptor gamma (PPARγ) is a master regulator in lipid metabolism and widely exists in vertebrates. However, the molecular structure and transcriptional activity of PPARγ in fish are still unclear. This study cloned PPARγ from Nile tilapia (Oreochromis niloticus) referred as NtPPARγ and transfected the NtPPARγ plasmids into(More)