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  • Silvia C Locatelli-Hoops, Fangmin C Sheen, Lioudmila Zoubak, Klaus Gawrisch, Alexei A. Yeliseev
  • Biology, Medicine
  • Protein expression and purification
  • 2013 (First Publication: 1 May 2013)
  • Expression of milligram quantities of functional, stable G protein-coupled receptors (GPCR) for high-resolution structural studies remains a challenging task. The goal of this work was to evaluateContinue Reading
  • Alexei A. Yeliseev, Lioudmila Zoubak, Thomas G. M. Schmidt
  • Chemistry, Medicine
  • Protein expression and purification
  • 2017 (First Publication: 1 March 2017)
  • Human cannabinoid receptor CB2 belongs to the class A of G protein-coupled receptor (GPCR). CB2 is predominantly expressed in membranes of cells of immune origin and is implicated in regulation ofContinue Reading
  • Niamh Ní Mhurchú, Lioudmila Zoubak, Gavin McGauran, Sara Linse, Alexei A. Yeliseev, David J. O’Connell
  • Medicine, Chemistry
  • Biochemistry
  • 2018 (First Publication: 31 July 2018)
  • The process of isolating recombinant G protein-coupled receptors from membrane preparations is challenging because the process requires solubilization in detergent micelles and multistep affinityContinue Reading
  • Ryan L Beckner, Lioudmila Zoubak, Kirk G. Hines, Klaus Gawrisch, Alexei A. Yeliseev
  • Medicine, Biology
  • The Journal of biological chemistry
  • 2019 (First Publication: 27 November 2019)
  • G protein-coupled receptors (GPCRs) comprise a large class of integral membrane proteins involved in the regulation of a broad spectrum of physiological processes and are a major target forContinue Reading