Lino A. Costa

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The global optimization of mixed integer non-linear problems (MINLP), constitutes a major area of research in many engineering applications. In this work, a comparison is made between an algorithm based on Simulated Annealing (M-SIMPSA) and two Evolutionary Algorithms: Genetic Algorithms (GAs) and Evolution Strategies (ESs). Results concerning the handling(More)
This paper presents a biped gait optimization system that combines bio-inspired Central Patterns Generators (CPGs) and a multi-objective evolutionary algorithm. CPGs are modeled as autonomous differential equations, that generate the necessary limb movements to perform the walking gait of a biped robot. The search for the best set of CPG parameters is(More)
Microfluidic devices can provide unique control over both the chemoattractant gradient and the migration environment of the cells. Our work incorporates laser-machined micro and nanofluidic channels into bulk fused silica and cover slip-sized silica wafers. We have designed “open” chemotaxis devices that produce passive chemoattractant gradients without an(More)
Microfluidic devices designed for chemotaxis assays were fabricated on fused silica substrates using femtosecond laser micromachining. These devices have built-in chemical concentration gradient forming structures and are ideally suited for establishing passive diffusion gradients over extended periods of time. Multiple gradient forming structures, with(More)
BACKGROUND The interaction of stem cells with their culture substrates is critical in controlling their fate and function. Declining stemness of adult-derived human mesenchymal stem cells (hMSCs) during in vitro expansion on tissue culture polystyrene (TCPS) severely limits their therapeutic efficacy prior to cell transplantation into damaged tissues. Thus,(More)
This letter presents a novel strategy for template synthesis of polymer structures with laser machined substrates. User-designed patterns of submicrometer holes with aspect ratios >10:1 and depths >10 μm were produced by focusing 160 fs, 5.2 μJ laser pulses on the surface of fused silica with a high numerical aperture microscope objective. Some holes were(More)
Requirement prioritization is a process that allows selection of the “key” candidate requirements, the ones that are the most important for the construction of quality and cost-controlled software. Requirement prioritization brings certain issues and challenges related with the different stakeholders involved in the project, as well as with(More)
Stromal cells in the tumor microenvironment play a key role in the metastatic properties of a tumor. It is recognized that cancer-associated fibroblasts (CAFs) and endothelial cells secrete factors capable of influencing tumor cell migration into the blood or lymphatic vessels. We developed a microfluidic device that can be used to image the interactions(More)