Lingxun Duan

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Plasmids construction Codons of human Pou5f1/Oct4 (NP_002692), Sox2 (NP_003097), Klf4 (NP_004226), and cMyc (NP_002458) were first optimized for high level protein expression in E. coli. They were then synthesized using DNA oligo based, PCR gene assembling method. Poly-arginine tag plus a linker sequence, i.e. ESGGGGSPGRRRRRRRRRRR, was added to each protein(More)
*Department of Cellular and Molecular Medicine, University of California at San Diego School of Medicine, La Jolla, CA 92093-0651; †Department of Medicine, Howard Hughes Medical Institute and University of California at San Diego School of Medicine, La Jolla, CA 92093; ‡Bioinformatics Graduate Program, University of California at San Diego, La Jolla, CA(More)
ChIP coupled with microarray provides a powerful tool to determine in vivo binding profiling of transcription factors to deduce regulatory circuitries in mammalian cells. Aiming at improving the specificity and sensitivity of such analysis, we developed a new technology called ChIP-DSL using the DNA selection and ligation (DSL) strategy, permitting robust(More)
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