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The dinoflagellate Prorocentrum minimum was successfully detected using loop-mediated isothermal amplification (LAMP) and real-time fluorescence quantitative PCR (RTFQ-PCR). Both specificity and sensitivity testing in the two methods have been validated. In the LAMP assay, the specific ladder-like pattern of bands only appeared in those templates containing(More)
The complete mitochondrial genome is of great importance for better understanding the genome-level characteristics and phylogenetic relationships among related species. In the present study, we determined the complete mitochondrial genome DNA sequence of the mud crab (Scylla paramamosain) by 454 deep sequencing and Sanger sequencing approaches. The complete(More)
The mud crab Scylla paramamosain plays a significant role in fishery resources in China. In this study, we developed 18 polymorphic microsatellite markers in this important crab by 5′ anchored PCR technique. A total of 125 alleles were detected in a single population of 32 individuals of S. paramamosain. The number of alleles per locus ranged from five to(More)
The mud crab (Scylla paramamosain) is a carnivorous portunid crab, mainly distributed along the southeastern coast of China. Mitochondrial DNA analysis in a previous study indicated a high level of genetic diversity and a low level of genetic differentiation. In this study, population genetic diversity and differentiation of S. paramamosain were(More)
Microsatellite markers from a transcriptome sequence library were initially isolated, and their genetic variation was characterized in a wild population of the mud crab (Scylla paramamosain). We then tested the association between these microsatellite markers and the growth performance of S. paramamosain. A total of 129 polymorphic microsatellite markers(More)
Nineteen microsatellite DNA markers of Collichthys lucidus were developed. The number of alleles for each locus ranged from three to 11. A total of 104 alleles were identified in 30 individuals collected from Zhejiang, China. The observed and expected heterozygosity per locus ranged from 0.2857 to 1.000 and from 0.5539 to 0.8774, respectively. Significant(More)
The red crab (Charybdis feriatus) is a large size marine portunid species, broadly distributed in the Indo–Pacific sea areas. In mainland China, it is naturally found in the coastal areas of Zhejiang, Fujian, Guangdong, Guangxi and Hainan provinces. The juvenile crab lives in the sandy shore, while the adult inhabits muddy offshore areas (Baylon and Suzuki(More)
Nibea albiflora is a commercially important fish species in China. Herein we report 16 novel polymorphic microsatellite markers in Nibea albiflora by using the 5′ anchored polymerase chain reaction (PCR) technique. The characteristics of these loci were estimated by using a sample of 30 individuals. A total of 79 alleles were detected with an average of 4.9(More)
Exon-primed intron-crossing (EPIC) markers have three advantages over anonymous genomic sequences in studying evolution of natural populations. First, the universal primers designed in exon regions can be applied across a broad taxonomic range. Second, the homology of EPIC-amplified sequences can be easily determined by comparing either their exon or intron(More)