Ling-xuan Mei

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OBJECTIVE To detect the MSX1 gene mutation in a Chinese family with oligodontia. METHODS Blood samples were obtained from seven affected and seven unaffected individuals in the pedigree. All exons and flanking intronic boundaries of the MSX1 gene were amplified with polymerase chain reaction technique and then directly sequenced. The website of(More)
OBJECTIVE To evaluate the effect of the osteoprotegerin (OPG) gene-modified autologous bone marrow stromal cells (BMSCs) on regeneration of periodontal defects, and to provide new experimental evidence to explore the gene therapy for periodontal disease. METHODS pSecTag2/B-opg was transduced into BMSCs by lipofectamine 2000. The expression of OPG protein(More)
PURPOSE In order to treat periodontitis by using tissue engineering and gene engineering technology, we constructed mammalian secreted expression pSecTag2/B-OPG vector, the cDNA sequence encoding osteoprotegerin(OPG) obtaining from 293 cell. METHODS The primers were designed based on the human OPG cDNA sequence. Total mRNA was isolated from 293 cells and(More)
OBJECTIVE To understand the effects of the IL-1, TNF-alpha cytokines on the pathogenesis of periapical lesions by investigating its gene expression in rat. METHODS The model was established by surgically exposing mandibular molar teeth and left open to permit infection from the oral environment. The SD rats were killed at 1, 2, 3, 4 weeks and mandibular(More)
OBJECTIVE In oder to treat periodontitis by using tissue engineering and gene engineering technology, the article established an transient expression system of bone marrow stromal cells (BMSC) modified by osteoprotegerin (OPG) gene and detected its expression using eukaryotic secreted expression pSecTag2/B-OPG plasmid. METHODS By solation and culture of(More)
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