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AIM To investigate the expression of toll-like receptor 4 (TLR4) and MD-2 gene and protein in Kupffer cells (KCs) and their role in ischemia-reperfusion (IR) injury of rat liver graft. METHODS KCs were isolated at 0 (control group), 2, 12, 24 h (IR group) following IR in rat liver graft. mRNA expression of TLR4 and MD-2 was detected by RT-PCR analysis,(More)
BACKGROUND Molecular genetic studies of Bombyx mori have led to profound advances in our understanding of the regulation of development. Bombyx mori brain, as a main endocrine organ, plays important regulatory roles in various biological processes. Microarray technology will allow the genome-wide analysis of gene expression patterns in silkworm brains. (More)
The development and application of the traditional GIS, as a platform, have long been heavyweight, expensive and non-public. Nowadays, with the explosion of Web2.0, they are facing challenge. An agile, lightweight, reusable GIS is now in need. This paper promotes a solution to meet this need. Based on SOA, the solution fulfills the concept of Web2.0 and(More)
Although the curative effects of bone marrow stromal cells (BMSCs) for acute kidney injury (AKI) have been recognized, their in vivo reparative capability is limited by the low levels of targeted homing and retention of intravenous injected cells. Stromal cell-derived factor-1 (SDF-1) plays an important role in stem cell homing and retention through(More)
Recent developments in high-throughput RNA sequencing (RNA-seq) technology have led to a dramatic impact on our understanding of the structure and expression profiles of the mammalian transcriptome. To gain insights into the usefulness of swine production and biomedical model, the transcriptome profiling of Rongchang pig brains and livers was characterized(More)
The chain of events resulting from oxidative stress is still barely understood. The hippocampus is particularly vulnerable to oxidative stress. With the emergence of high-throughput sequencing, many different biological components can be discovered simultaneously. Using RNA-seq technology, we conducted a whole-transcriptome analysis of the hippocampi of(More)
Recent evidence identifies the hippocampus, a brain structure commonly associated with learning and memory, as key to the regulation of food intake and the development and consequences of obesity. Intake of a high fat diet (HFD) results in altered consumptive behavior, hippocampal damage, and cognitive deficits. While many studies report the effects of HFD(More)
Eye detection is the preconditions for fatigue driving detection. In this paper, considering the deficiency of the origin algorithm based on rank order filter, an improved eye detection algorithm based on rank order filter and projection function is proposed. At first, according to the structure features and gray features, an ellipse mask combined with rank(More)
Livestock and poultry farming is a major source of agricultural pollution. However, our knowledge of the constraining factors of the geographic distribution of pollutants from livestock and poultry farming is still limited. In this study, using the optimized pollutant generation coefficients, we estimated the annual pollutant productions of eight livestock(More)