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A palette of fluorescent proteins optimized for diverse cellular environments
To perform quantitative live cell imaging, investigators require fluorescent reporters that accurately report protein localization and levels, while minimally perturbing the cell. Yet, within theExpand
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Superfolder GFP Is Fluorescent in Oxidizing Environments When Targeted via the Sec Translocon
The ability to study proteins in live cells using genetically encoded fluorescent proteins (FPs) has revolutionized cell biology (1–3). Researchers have created numerous FP biosensors and optimizedExpand
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The Phagocytosis and Toxicity of Amorphous Silica
Background Inhalation of crystalline silica is known to cause an inflammatory reaction and chronic exposure leads to lung fibrosis and can progress into the disease, silicosis. Cultured macrophagesExpand
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Assessing the Tendency of Fluorescent Proteins to Oligomerize Under Physiologic Conditions
Several fluorescent proteins (FPs) are prone to forming low‐affinity oligomers. This undesirable tendency is exacerbated when FPs are confined to membranes or when fused to naturally oligomericExpand
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Probing endoplasmic reticulum dynamics using fluorescence imaging and photobleaching techniques.
This unit describes approaches and tools for studying the dynamics and organization of endoplasmic reticulum (ER) membranes and proteins in living cells using fluorescence microscopy. The ER plays aExpand
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Fluorescent proteins in cellular organelles: serious pitfalls and some solutions.
Fluorescent proteins (FPs) have been powerful tools for cell biologists for over 15 years. The large variety of FPs available rarely comes with an instruction manual or a warning label. The potentialExpand
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Large-scale, cross-flow based isolation of highly pure and endocytosis-competent extracellular vesicles
ABSTRACT Isolation of extracellular vesicles (EVs) from cell culture supernatant or plasma can be accomplished in a variety of ways. Common measures to quantify relative success are: concentration ofExpand
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Engineering and exploitation of a fluorescent HIV-1 gp120 for live cell CD4 binding assays.
The HIV-1 envelope glycoprotein, gp120, binds the host cell receptor, CD4, in the initial step of HIV viral entry and infection. This process is an appealing target for the development of inhibitoryExpand
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Alcohol disrupts endoplasmic reticulum function and protein secretion in hepatocytes.
BACKGROUND Many alcoholic patients have serum protein deficiency that contributes to their systemic problems. The unfolded protein response (UPR) is induced in response to disequilibrium in theExpand
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Cysteineless non-glycosylated monomeric blue fluorescent protein, secBFP2, for studies in the eukaryotic secretory pathway.
Fluorescent protein (FP) technologies suitable for use within the eukaryotic secretory pathway are essential for live cell and protein dynamic studies. Localization of FPs within the endoplasmicExpand
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