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We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted… (More)
The history, benefits, and drawbacks to pure rule-based programming is discussed. A simple extension to pure rule-based programming is described. The extensions are very quick to code and can be easily customized to support a range of knowledge engineering applications.
The distribution of light in tissues of varying blood content has been modeled using a gel agar system as a tissue phantom. Hematoporphyrin derivative (Photofrin I or HpD) was suspended in gels, along with a chemical indicator of its cytotoxic intermediate (singlet oxygen), and various amounts of red blood cells. The singlet oxygen detector used was… (More)