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We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted… (More)
The history, benefits, and drawbacks to pure rule-based programming is discussed. A simple extension to pure rule-based programming is described. The extensions are very quick to code and can be easily customized to support a range of knowledge engineering applications.