Linda J. Osborne

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Various combinations of cytokines have profoundly different effects on inhibition of apoptosis and stimulation of self-renewal division of hematopoietic stem cells (HSC) in short-term, ex vivo culture. Our goal was to quantitate expansion of cells with a primitive CD34+ Thy-1+ phenotype, as well as cell cycling, division history, differentiation, and(More)
Adipose cytosol treated with spermine showed an aggregation of a cytosolic component which was isolated by centrifugation at 16,000 X g for 20 min. The resultant pellet contained 10% of protein, 40% of lipid and over 75-97% of Mg2+-dependent phosphatidate phosphohydrolase and CTP:phosphocholine cytidylyltransferase activities present in the original(More)
The effect of Z protein, bovine serum albumin and spermine on 1,2-diacylglycerol acyltransferase (DGAT) was investigated. DGAT was measured in the presence of [14C]-palmitoyl-CoA and 1,2-diacylglycerol dispersed in Tween 20. As noted earlier, the activation of DGAT was observed in the presence of either spermine or Mg2+, with spermine being more effective.(More)
Adipose glycerolipid formation was studied in relation to age, site of adipose tissue and cell size in developing rats. Glycerolipid formation was measured in vitro in the presence of [14C]glycerol 3-phosphate, palmitate, ATP, CoA and Mg2+ by using adipocyte homogenates derived from various age groups of animals. In the epididymis fat pads, glycerolipid(More)
A simple one-step filtration method is described to separate larger adipocytes from the smaller ones by using nylon screen (52 microM pore size). Adipocytes retained on the screen were larger (60-90 micrometers) compared with those that passed through the screen. By using this separation technique, activities of various enzymes involved in triacylglycerol(More)
Thioredoxin expression within the reproductive tissues of the female mouse was analysed during the oestrous cycle stages of dioestrus, oestrus and metoestrus by Western blot analyses and immunocytochemistry. From Western blot analyses the expression of thioredoxin was found to be increased in oestrus compared to dioestrus and metoestrus. Localisation of(More)
The conversion of [14C]-labeled compounds such as acetate, glucose, pyruvate and palmitate into CO2, glyceride-glycerol, glyceride fatty acids and total lipids was monitored in the average and matching adipocyte (with respect to size) preparations from young (6–9 wk) and old (age 56–60 wk) male Sprague-Dawley rats. The average cell size populations from(More)
The inability to cultivate Mycobacterium leprae in vitro has been a major bottleneck in leprosy research. There have been numerous reports on successful in vitro cultivation of this organism, but these reports could not be confirmed by others in the field. Hence, in vitro multiplication of M. leprae was evaluated in various culture media. Only 2 media(More)
[14C]Acetate incorporation into different lipid fractions was measured as a function of adipocyte size by using the larger and smaller adipocytes derived from Sprague-Dawley rats. In both the larger and smaller adipocytes, [14C]acetate was incorporated into phospholipid, diacylglycerol, free fatty acid and triacylglycerol fractions. Although the rates of(More)
The properties of Mg2+-dependent and Mg2+-independent phosphatidate phosphohydrolase activities were investigated in different subcellular fractions in rat adipose tissue. Phosphatidate phosphohydrolase activity was measured in the presence of aqueous dispersed phosphatidate as substrate, and the release of inorganic phosphate was taken as a measure of(More)